1Genomics 2003 May 81: 510-8
TitleHigh-resolution SNP scan of chromosome 6p21 in pooled samples from patients with complex diseases.
AbstractWe apply a high-throughput protocol of chip-based mass spectrometry (matrix-assisted laser desorption/ionization time-of-flight; MALDI-TOF) as a method of screening for differences in single-nucleotide polymorphism (SNP) allele frequencies. Using pooled DNA from individuals with asthma, Crohn's disease (CD), schizophrenia, type 1 diabetes (T1D), and controls, we selected 534 SNPs from an initial set of 1435 SNPs spanning a 25-Mb region on chromosome 6p21. The standard deviations of measurements of time of flight at different dots, from different PCRs, and from different pools indicate reliable results on each analysis step. In 90% of the disease-control comparisons we found allelic differences of <10%. Of the T1D samples, which served as a positive control, 10 SNPs with significant differences were observed after taking into account multiple testing. Of these 10 SNPs, 5 are located between DQB1 and DRB1, confirming the known association with the DR3 and DR4 haplotypes whereas two additional SNPs also reproduced known associations of T1D with DOB and LTA. In the CD pool also, two earlier described associations were found with SNPs close to DRB1 and MICA. Additional associations were found in the schizophrenia and asthma pools. They should be confirmed in individual samples or can be used to develop further quality criteria for accepting true differences between pools. The determination of SNP allele frequencies in pooled DNA appears to be of value in assigning further genotyping priorities also in large linkage regions.
SCZ Keywordsschizophrenia
2Subst Use Misuse 2003 Dec 38: 2097-107
TitleClinician attributions and disease model perspectives of mentally ill, chemically addicted patients: a preliminary investigation.
AbstractBrickman et al.'s (Brickman, P., Rabinowitz, V. C., Coates, D., Cohn, E., Kidder, L. (1982). Models of helping and coping. American Psychologist 37:364-384.) models of helping and coping provided a framework by which to compare clinicians' attributions of blame and control among several hypothetical patients. Sixty-one mental health clinicians (MHCs) and addiction clinicians (ACs)--mostly master's level clinicians and registered nurses--rated attributions toward vignettes that depicted individuals with schizophrenia, alcoholism, and mentally ill, cheMICAlly addicted (MICA) classifications in 1995. Results indicate that MHCs attributed more blame to MICA patients than did ACs, but did not differ on their attributions of control. MHCs' and ACs' attributions of blame and control were generally low, consistent with a medical model. However, the endorsement of a disease model of alcoholism did not significantly predict the amount of blame attributed by the clinicians. Implications for treatment planning for MICA patients are discussed.
SCZ Keywordsschizophrenia