mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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Gene Summary

Ligand Binding Site Mutation Information

Protein Structure Related Information

Gene Expression and Gene-Gene Network

Phenotype Information

Pharmacological Information

Conservation Information for LBS

Gene summary for MELK
Gene summary
Basic gene Info.Gene symbolMELK
Gene namematernal embryonic leucine zipper kinase
SynonymsHPK38
CytomapUCSC genome browser: 9p13.2
Type of geneprotein-coding
RefGenesNM_001256685.1,
NM_001256687.1,NM_001256688.1,NM_001256689.1,NM_001256690.1,
NM_001256691.1,NM_001256692.1,NM_001256693.1,NM_014791.3,
NR_046337.1,
DescriptionpEg3 kinaseprotein kinase Eg3protein kinase PK38tyrosine-protein kinase MELK
Modification date20141207
dbXrefs MIM : 607025
HGNC : HGNC
Ensembl : ENSG00000165304
HPRD : 06119
Vega : OTTHUMG00000019906
ProteinUniProt: Q14680
go to UniProt's Cross Reference DB Table
ExpressionCleanEX: HS_MELK
BioGPS: 9833
PathwayNCI Pathway Interaction Database: MELK
KEGG: MELK
REACTOME: MELK
Pathway Commons: MELK
ContextiHOP: MELK
ligand binding site mutation search in PubMed: MELK
UCL Cancer Institute: MELK
Assigned class in mutLBSgeneDBB: This gene belongs to targetable_mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez
GO IDGO TermPubMed ID
GO:0006915apoptotic process17280616
GO:0008283cell proliferation17280616
GO:0018108peptidyl-tyrosine phosphorylation16216881
GO:0046777protein autophosphorylation16216881


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Ligand binding site mutations for MELK
Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)
 : non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency
LBSAAchange of nsSNVCancer type# samples
L139L139PCOAD1
V25V25IUCEC1
cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.


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Protein structure related information for MELK
Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
  (ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)
: nsSNV at non-LBS: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.
LBSAAchange of nsSNVRelative stability change
V25V25I0.16681873
L139L139P-1.1584906
(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for MELK from PDB

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Differential gene expression and gene-gene network for MELK
Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)
* Left PPI network was created from samples with mutations in the LBS of MELK and the right PPI network was created from samples without mutations in the LBS of MELK. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.


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Phenotype information for MELK
Gene level disease information (DisGeNet)
Disease IDDisease name# PubMedAssociation type
umls:C0017661Glomerulonephritis, IGA1Biomarker

Mutation level pathogenic information (ClinVar annotation)
Allele IDAA changeClinical significanceOriginPhenotype IDs

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Pharmacological information for MELK
Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network
Drug information targeting mutLBSgene (Approved drugs only)
Drug statusDrugBank IDNameTypeDrug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of MELK go to BioLip
Ligand IDLigand short nameLigand long namePDB IDPDB namemutLBS
1WSN-(3-AMINOPROPYL)-8-[(3-FLUOROPHENYL)AMINO]- 2,4,5,7-TETRAHYDROPYRAZOLO[3,4-E]INDAZOLE-3- CARBOXAMIDE4bkzAL139
6Z77-({4-[(3-HYDROXY-5-METHOXYPHENYL)AMINO] BENZOYL}AMINO)-1,2,3,4- TETRAHYDROISOQUINOLINIUM4d2pAL139
6Z77-({4-[(3-HYDROXY-5-METHOXYPHENYL)AMINO] BENZOYL}AMINO)-1,2,3,4- TETRAHYDROISOQUINOLINIUM4d2pBL139
6Z77-({4-[(3-HYDROXY-5-METHOXYPHENYL)AMINO] BENZOYL}AMINO)-1,2,3,4- TETRAHYDROISOQUINOLINIUM4d2pCL139
6Z77-({4-[(3-HYDROXY-5-METHOXYPHENYL)AMINO] BENZOYL}AMINO)-1,2,3,4- TETRAHYDROISOQUINOLINIUM4d2pDL139
NF54-BROMO-N-(2,3,4,5-TETRAHYDRO-1H-3- BENZAZEPIN-7-YL)BENZAMIDE4d2wAL139
NF54-BROMO-N-(2,3,4,5-TETRAHYDRO-1H-3- BENZAZEPIN-7-YL)BENZAMIDE4d2wCL139
NF54-BROMO-N-(2,3,4,5-TETRAHYDRO-1H-3- BENZAZEPIN-7-YL)BENZAMIDE4d2wDL139
5QM3-(ISOQUINOLIN-7-YL)PROP-2-YN-1-OL4umpAL139
B5S3-{5-[(3-HYDROXY-5-METHOXYPHENYL)AMINO]-2- (PHENYLCARBAMOYL)PHENOXY}PROPAN-1-AMINIUM4umqAL139
3I73-[2-(PHENYLCARBAMOYL)-5-(1H-PYRAZOL-4-YL) PHENOXY]PROPAN-1-AMINIUM4d2tDL139
47W1-(4-{[3-(ISOQUINOLIN-7-YL)PROP-2-YN-1-YL] OXY}-2-METHOXYBENZYL)PIPERAZINEDIIUM4umtAL139
0V0(2-ETHOXY-4-{[3-(ISOQUINOLIN-7-YL)PROP-2-YN- 1-YL]OXY}PHENYL)METHANAMINIUM4umuAL139
3I73-[2-(PHENYLCARBAMOYL)-5-(1H-PYRAZOL-4-YL) PHENOXY]PROPAN-1-AMINIUM4d2tAV25
45R7-{[2-METHOXY-4-(1H-PYRAZOL-4-YL)BENZOYL] AMINO}-2,3,4,5-TETRAHYDRO-1H-3-BENZAZEPINIUM4d2vAV25
45R7-{[2-METHOXY-4-(1H-PYRAZOL-4-YL)BENZOYL] AMINO}-2,3,4,5-TETRAHYDRO-1H-3-BENZAZEPINIUM4d2vDV25
ANPAMP-PNP4bl1AV25 L139
82B3'-{[(4-BROMO-1-METHYL-1H-PYRROL-2-YL) CARBONYL]AMINO}-N-[(1S)-1-PHENYL-2-(PYRROLIDIN-1-YL) ETHYL]-1',4'-DIHYDRO-5'H-SPIRO[CYCLOPROPANE- 1,6'-PYRROLO[3,4-C]PYRAZOLE]-5'-CARBOXAMIDE4bkyAV25 L139
5QM3-(ISOQUINOLIN-7-YL)PROP-2-YN-1-OL4umpCV25 L139
5QM3-(ISOQUINOLIN-7-YL)PROP-2-YN-1-OL4umpDV25 L139
QBB4-FLUORO-N-(1,2,3,4-TETRAHYDROISOQUINOLIN-7- YL)BENZAMIDE4umrAV25 L139
45R7-{[2-METHOXY-4-(1H-PYRAZOL-4-YL)BENZOYL] AMINO}-2,3,4,5-TETRAHYDRO-1H-3-BENZAZEPINIUM4d2vCV25 L139


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Conservation information for LBS of MELK
Multiple alignments for Q14680 in multiple species
LBSAA sequence# speciesSpecies
A129HSQGYAHRDLK5Homo sapiens, Caenorhabditis elegans, Danio rerio, Xenopus tropicalis, Mus musculus
A129HRDGIAHRDVK1Arabidopsis thaliana
A129HQDGVAHRDVK1Arabidopsis thaliana
A23GTGGFAKVKLA3Homo sapiens, Xenopus tropicalis, Mus musculus
A23GSGSFAKVHVA1Arabidopsis thaliana
A23GSGSFAKVHLA1Arabidopsis thaliana
A23GSGGFGKVRLA1Caenorhabditis elegans
A23GSGGFAKVKLG1Danio rerio
A38TGEMVAIKIMD2Homo sapiens, Mus musculus
A38TGELVAIKIID1Arabidopsis thaliana
A38SDELVAVKIIE1Arabidopsis thaliana
A38TNQKVAIKIID1Caenorhabditis elegans
A38TGEKVAIKIME1Danio rerio
A38TGEKVAIKIMD1Xenopus tropicalis
A60KTEIEALKNLR1Homo sapiens
A60IREIEAMRRLH1Arabidopsis thaliana
A60IREIDAMRRLR1Arabidopsis thaliana
A60QTEMDALRNLS1Caenorhabditis elegans
A60KIEIEAMKNLS1Danio rerio
A60KTEIDAMKNLS1Xenopus tropicalis
A60KTEIDALKSLR1Mus musculus
C70-HQHICQLYHV2Homo sapiens, Mus musculus
C70-HQHVCRLYHV2Danio rerio, Xenopus tropicalis
C70NHPNVLKIHEV1Arabidopsis thaliana
C70HHPNILKIHEV1Arabidopsis thaliana
C70-HQNICRLYHY1Caenorhabditis elegans
C89MVLEYCPGGEL4Homo sapiens, Danio rerio, Xenopus tropicalis, Mus musculus
C89LVVEYAAGGEL1Arabidopsis thaliana
C89LVMELASGGEL1Arabidopsis thaliana
C89IVMEYCSGGEM1Caenorhabditis elegans
D150KLKLIDFGLCA2Homo sapiens, Mus musculus
D150NLKVSDFGLSA2Arabidopsis thaliana, Arabidopsis thaliana
D150NLKLIDFGLCA2Danio rerio, Xenopus tropicalis
D150HLKLIDFGLCA1Caenorhabditis elegans
E136RDLKPENLLFD2Homo sapiens, Mus musculus
E136RDVKPQNLLLD2Arabidopsis thaliana, Arabidopsis thaliana
E136RDLKPENLLID2Danio rerio, Xenopus tropicalis
E136RDLKPENLLLT1Caenorhabditis elegans
E15YYELHETIGTG2Homo sapiens, Xenopus tropicalis
E15KYELGRRLGSG2Arabidopsis thaliana, Arabidopsis thaliana
E15FYAVHDELGSG1Caenorhabditis elegans
E15HYEVYETIGSG1Danio rerio
E15YYELYETIGTG1Mus musculus
E57PRIKTEIEALK1Homo sapiens
E57PRIIREIEAMR1Arabidopsis thaliana
E57PRIIREIDAMR1Arabidopsis thaliana
E57PRVQTEMDALR1Caenorhabditis elegans
E57PRVKIEIEAMK1Danio rerio
E57PRVKTEIDAMK1Xenopus tropicalis
E57PRVKTEIDALK1Mus musculus
E87IFMVLEYCPGG3Homo sapiens, Xenopus tropicalis, Mus musculus
E87IYLVVEYAAGG1Arabidopsis thaliana
E87IYLVMELASGG1Arabidopsis thaliana
E87FFIVMEYCSGG1Caenorhabditis elegans
E87IYMVLEYCPGG1Danio rerio
E93YCPGGELFDYI4Homo sapiens, Danio rerio, Xenopus tropicalis, Mus musculus
E93YAAGGELFTKL1Arabidopsis thaliana
E93LASGGELFSKV1Arabidopsis thaliana
E93YCSGGEMFDYI1Caenorhabditis elegans
F151LKLIDFGLCAK5Homo sapiens, Caenorhabditis elegans, Danio rerio, Xenopus tropicalis, Mus musculus
F151LKVSDFGLSAL2Arabidopsis thaliana, Arabidopsis thaliana
F22IGTGGFAKVKL3Homo sapiens, Xenopus tropicalis, Mus musculus
F22LGSGSFAKVHV1Arabidopsis thaliana
F22LGSGSFAKVHL1Arabidopsis thaliana
F22LGSGGFGKVRL1Caenorhabditis elegans
F22IGSGGFAKVKL1Danio rerio
G18LHETIGTGGFA2Homo sapiens, Xenopus tropicalis
G18LGRRLGSGSFA2Arabidopsis thaliana, Arabidopsis thaliana
G18VHDELGSGGFG1Caenorhabditis elegans
G18VYETIGSGGFA1Danio rerio
G18LYETIGTGGFA1Mus musculus
G20ETIGTGGFAKV3Homo sapiens, Xenopus tropicalis, Mus musculus
G20RRLGSGSFAKV2Arabidopsis thaliana, Arabidopsis thaliana
G20DELGSGGFGKV1Caenorhabditis elegans
G20ETIGSGGFAKV1Danio rerio
G21TIGTGGFAKVK3Homo sapiens, Xenopus tropicalis, Mus musculus
G21RLGSGSFAKVH2Arabidopsis thaliana, Arabidopsis thaliana
G21ELGSGGFGKVR1Caenorhabditis elegans
G21TIGSGGFAKVK1Danio rerio
G92EYCPGGELFDY4Homo sapiens, Danio rerio, Xenopus tropicalis, Mus musculus
G92EYAAGGELFTK1Arabidopsis thaliana
G92ELASGGELFSK1Arabidopsis thaliana
G92EYCSGGEMFDY1Caenorhabditis elegans
H130SQGYAHRDLKP5Homo sapiens, Caenorhabditis elegans, Danio rerio, Xenopus tropicalis, Mus musculus
H130RDGIAHRDVKP1Arabidopsis thaliana
H130QDGVAHRDVKP1Arabidopsis thaliana
I149HKLKLIDFGLC2Homo sapiens, Mus musculus
I149GNLKVSDFGLS2Arabidopsis thaliana, Arabidopsis thaliana
I149LHLKLIDFGLC1Caenorhabditis elegans
I149HNLKLIDFGLC1Danio rerio
I149QNLKLIDFGLC1Xenopus tropicalis
I17ELHETIGTGGF2Homo sapiens, Xenopus tropicalis
I17ELGRRLGSGSF2Arabidopsis thaliana, Arabidopsis thaliana
I17AVHDELGSGGF1Caenorhabditis elegans
I17EVYETIGSGGF1Danio rerio
I17ELYETIGTGGF1Mus musculus
I69R-HQHICQLYH2Homo sapiens, Mus musculus
I69S-HQHVCRLYH2Danio rerio, Xenopus tropicalis
I69HNHPNVLKIHE1Arabidopsis thaliana
I69RHHPNILKIHE1Arabidopsis thaliana
I69S-HQNICRLYH1Caenorhabditis elegans
K40EMVAIKIMDK-2Homo sapiens, Mus musculus
K40ELVAIKIIDKQ1Arabidopsis thaliana
K40ELVAVKIIEKK1Arabidopsis thaliana
K40QKVAIKIIDK-1Caenorhabditis elegans
K40EKVAIKIMEK-1Danio rerio
K40EKVAIKIMDK-1Xenopus tropicalis
L139KPENLLFDEYH1Homo sapiens
L139KPQNLLLDKQG1Arabidopsis thaliana
L139KPQNLLLDEQG1Arabidopsis thaliana
L139KPENLLLTEDL1Caenorhabditis elegans
L139KPENLLIDEDH1Danio rerio
L139KPENLLIDEDQ1Xenopus tropicalis
L139KPENLLFDENH1Mus musculus
L148QGNLKVSDFGL2Arabidopsis thaliana, Arabidopsis thaliana
L148YHKLKLIDFGL1Homo sapiens
L148DLHLKLIDFGL1Caenorhabditis elegans
L148DHNLKLIDFGL1Danio rerio
L148DQNLKLIDFGL1Xenopus tropicalis
L148NHKLKLIDFGL1Mus musculus
L27FAKVKLACHIL1Homo sapiens
L27FAKVHVARSIS1Arabidopsis thaliana
L27FAKVHLARSIE1Arabidopsis thaliana
L27FGKVRLATHLL1Caenorhabditis elegans
L27FAKVKLGRHKL1Danio rerio
L27FAKVKLASHLT1Xenopus tropicalis
L27FAKVKLACHVL1Mus musculus
L61TEIEALKNLR-1Homo sapiens
L61REIEAMRRLHN1Arabidopsis thaliana
L61REIDAMRRLRH1Arabidopsis thaliana
L61TEMDALRNLS-1Caenorhabditis elegans
L61IEIEAMKNLS-1Danio rerio
L61TEIDAMKNLS-1Xenopus tropicalis
L61TEIDALKSLR-1Mus musculus
L64EALKNLR-HQH1Homo sapiens
L64EAMRRLHNHPN1Arabidopsis thaliana
L64DAMRRLRHHPN1Arabidopsis thaliana
L64DALRNLS-HQN1Caenorhabditis elegans
L64EAMKNLS-HQH1Danio rerio
L64DAMKNLS-HQH1Xenopus tropicalis
L64DALKSLR-HQH1Mus musculus
L86KIFMVLEYCPG3Homo sapiens, Xenopus tropicalis, Mus musculus
L86KIYLVVEYAAG1Arabidopsis thaliana
L86KIYLVMELASG1Arabidopsis thaliana
L86KFFIVMEYCSG1Caenorhabditis elegans
L86KIYMVLEYCPG1Danio rerio
N137DLKPENLLFDE2Homo sapiens, Mus musculus
N137DLKPENLLIDE2Danio rerio, Xenopus tropicalis
N137DVKPQNLLLDK1Arabidopsis thaliana
N137DVKPQNLLLDE1Arabidopsis thaliana
N137DLKPENLLLTE1Caenorhabditis elegans
P90VLEYCPGGELF4Homo sapiens, Danio rerio, Xenopus tropicalis, Mus musculus
P90VVEYAAGGELF1Arabidopsis thaliana
P90VMELASGGELF1Arabidopsis thaliana
P90VMEYCSGGEMF1Caenorhabditis elegans
R53SGMEPRIIREI2Arabidopsis thaliana, Arabidopsis thaliana
R53SDL-PRIKTEI1Homo sapiens
R53HDL-PRVQTEM1Caenorhabditis elegans
R53DDL-PRVKIEI1Danio rerio
R53DDL-PRVKTEI1Xenopus tropicalis
R53SDL-PRVKTEI1Mus musculus
T16YELHETIGTGG2Homo sapiens, Xenopus tropicalis
T16YELGRRLGSGS2Arabidopsis thaliana, Arabidopsis thaliana
T16YAVHDELGSGG1Caenorhabditis elegans
T16YEVYETIGSGG1Danio rerio
T16YELYETIGTGG1Mus musculus
T19HETIGTGGFAK2Homo sapiens, Xenopus tropicalis
T19GRRLGSGSFAK2Arabidopsis thaliana, Arabidopsis thaliana
T19HDELGSGGFGK1Caenorhabditis elegans
T19YETIGSGGFAK1Danio rerio
T19YETIGTGGFAK1Mus musculus
V25GGFAKVKLACH2Homo sapiens, Mus musculus
V25GSFAKVHVARS1Arabidopsis thaliana
V25GSFAKVHLARS1Arabidopsis thaliana
V25GGFGKVRLATH1Caenorhabditis elegans
V25GGFAKVKLGRH1Danio rerio
V25GGFAKVKLASH1Xenopus tropicalis
Y88FMVLEYCPGGE3Homo sapiens, Xenopus tropicalis, Mus musculus
Y88YLVVEYAAGGE1Arabidopsis thaliana
Y88YLVMELASGGE1Arabidopsis thaliana
Y88FIVMEYCSGGE1Caenorhabditis elegans
Y88YMVLEYCPGGE1Danio rerio


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