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  2-cell blastomere

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in 2-cell blastomere

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160122Illumina HiSeq 2000Homo sapienshuman 2-cell 62-cell blastomere
GSE44183GSM1160121Illumina HiSeq 2000Homo sapienshuman 2-cell 52-cell blastomere
GSE44183GSM1160120Illumina HiSeq 2000Homo sapienshuman 2-cell 12-cell blastomere

Gene rank in 2-cell blastomere

Displaying 14611-14620 of 14758 results.
Rank orderGene SymbolEnsembl ID
14611FGF13ENSG00000129682
14612GABRQENSG00000268089
14613GAGE1ENSG00000205777
14614GAGE12JENSG00000224659
14615GAGE2AENSG00000189064
14616GDPD2ENSG00000130055
14617GJB1ENSG00000169562
14618GLRA4ENSG00000188828
14619GPR119ENSG00000147262
14620GPR50ENSG00000102195