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  Breast Cancer cell line (SKBR3)

Overall DesignFirst, hand-picked single cells are lysed and reverse transcribed using a poly-A primer including cell-specific barcodes, a 5' Illumina adapter and a T7 promoter overhang to convert mRNA to single stranded cDNA (ss cDNA). The gDNA and single stranded cDNA are then subjected to quasilinear whole genome amplification, as previously described, using an adapter with a defined 27 nucleotide sequence at the 5’ end followed by 8 random nucleotides. After 7 rounds of amplification, the gDNA and cDNA are copied to generate a variety of different short amplicon (0.5–2.5 kb) species, with a majority of amplicons containing adapter Ad-2 at both ends and a small fraction of cDNA derived amplicons containing Ad-2 at one end and Ad-1x at the other. Next, the sample is split into two tubes to further amplify gDNA and cDNA. The tube used to sequence gDNA is amplified using PCR. Following sonication, adapter Ad-2 removal, and cell-specific indexed Illumina library preparation, this half is used to sequence gDNA. The tube used to sequence cDNA is converted to double-stranded cDNA and amplified using in vitro transcription such that the amplified RNA (aRNA) is uniquely produced from cDNA but not gDNA. 3’ Illumina adapters are then ligated to the aRNA followed by reverse transcription and PCR, allowing quantification of mRNA.
SummarySingle-cell genomics and single-cell transcriptomics have recently emerged as powerful tools to study the biology of single cells at a genome-wide scale. Here we describe a method that allows the integration of genomic DNA and mRNA sequencing from the same cell. We use this method to correlate DNA copy number variation to transcriptome variability among individual cells.
Dataset viewGSE62952
PMID25599178

Samples in Breast Cancer cell line (SKBR3)

Displaying 1-10 of 21 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE62952GSM1537130.1Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.3Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.6Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.7Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.9Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.10Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.12Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.13Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.15Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)
GSE62952GSM1537130.17Illumina HiSeq 2500Homo sapienssingle cell mRNA sequencing data from single SKBR3 cells obtained with the DR-seq protocolBreast Cancer cell line (SKBR3)

Gene rank in Breast Cancer cell line (SKBR3)

Displaying 12081-12090 of 12151 results.
Rank orderGene SymbolEnsembl ID
12081SATB2ENSG00000119042
12082SDSLENSG00000139410
12083SEC14L1P1ENSG00000213693
12084SH3BGRL2ENSG00000198478
12085SLC25A34ENSG00000162461
12086SLC25A41ENSG00000181240
12087SLC31A2ENSG00000136867
12088SYDE2ENSG00000097096
12089SYT16ENSG00000139973
12090SYT5ENSG00000129990