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  Cell line KBM7, derived from a patient with chronic myeloid leukemia

Overall DesignIn this dataset, single-cell mRNA sequencing results from 96 single KBM7 cells have been deposited
SummaryMammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large Lamina Associated Domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of ~400 maps reveals a core architecture of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts are more sensitive to a change in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, single-cell gene expression and chromatin accessibility analysis shows that loci with consistent NL contacts are expressed at lower levels and are more consistently inaccessible than loci with lower contact frequencies. These results highlight fundamental principles of single cell chromatin organization.
Dataset viewGSE68596
PMID26365489

Samples in Cell line KBM7, derived from a patient with chronic myeloid leukemia

Displaying 21-30 of 96 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE68596GSM1676569.21Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.22Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.23Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.24Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.25Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.26Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.27Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.28Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.29Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia
GSE68596GSM1676569.30Illumina HiSeq 2500Homo sapiensSingle cell mRNA sequencing data from KMB7 cellsCell line KBM7, derived from a patient with chronic myeloid leukemia

Gene rank in Cell line KBM7, derived from a patient with chronic myeloid leukemia

Displaying 1-10 of 13608 results.
Rank orderGene SymbolEnsembl ID
1LOC100505876
2ERC2-IT1ENSG00000281708
3SLITRK4__chrX
4CLEC4GP1ENSG00000268297
5MIR1244-1
6MIR1244-2ENSG00000283498
7MIR1244-3ENSG00000283429
8SLC25A37ENSG00000147454
9KCNJ3ENSG00000162989
10EEF1A1ENSG00000156508