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  GM12878

Overall DesignSingle GM12878 cells were picked and RNA-seq libraries were generated using the SMART-seq protocol. We also carried out RNA-seq experiments on pools of 10, 30 and 100 cells, on 100pg and 10ng of total RNA, and on pools of 10 cells that were subsequently split into 10 separate sample and processed as if they were single cells in order to assess technical variation in our experiments.
SummaryRNA-seq transcriptome measurements are typically performed by isolating RNA from large numbers of cells in culture or tissues. While highly informative, such experiments mask the variability in gene expression patterns that exists between individual cells. To gain insight into the dynamics of gene expression on the level of single-cells, we have carried out the transcriptomes of single-cells from the GM12878 cell line using RNA-seq.
Dataset viewGSE44618
PMID24299736

Samples in GM12878

Displaying 11-20 of 28 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44618GSM1087872Illumina HiSeq 2000Homo sapiens12541-100mers-GM12878_193-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087873Illumina HiSeq 2000Homo sapiens12542-100mers-GM12878_194-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087874Illumina HiSeq 2000Homo sapiens12543-100mers-GM12878_195-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087875Illumina HiSeq 2000Homo sapiens12818-100mers-GM12878_200-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087876Illumina HiSeq 2000Homo sapiens12819-100mers-GM12878_205-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087877Illumina HiSeq 2000Homo sapiens12820-100mers-GM12878_208-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087882Illumina HiSeq 2000Homo sapiens13278-100mers-GM12878-204-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087883Illumina HiSeq 2000Homo sapiens13279-100mers-GM12878-207-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087884Illumina HiSeq 2000Homo sapiens13282-100mers-GM12878-235-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087885Illumina HiSeq 2000Homo sapiens13283-100mers-GM12878-236-TopHat-1.4.1-GENCODE-V13GM12878

Gene rank in GM12878