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  GM12878

Overall DesignSingle GM12878 cells were picked and RNA-seq libraries were generated using the SMART-seq protocol. We also carried out RNA-seq experiments on pools of 10, 30 and 100 cells, on 100pg and 10ng of total RNA, and on pools of 10 cells that were subsequently split into 10 separate sample and processed as if they were single cells in order to assess technical variation in our experiments.
SummaryRNA-seq transcriptome measurements are typically performed by isolating RNA from large numbers of cells in culture or tissues. While highly informative, such experiments mask the variability in gene expression patterns that exists between individual cells. To gain insight into the dynamics of gene expression on the level of single-cells, we have carried out the transcriptomes of single-cells from the GM12878 cell line using RNA-seq.
Dataset viewGSE44618
PMID24299736

Samples in GM12878

Displaying 1-10 of 28 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44618GSM1087862Illumina HiSeq 2000Homo sapiens12522-100mers-GM12878_183-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087863Illumina HiSeq 2000Homo sapiens12523-100mers-GM12878_184-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087864Illumina HiSeq 2000Homo sapiens12524-100mers-GM12878_185-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087865Illumina HiSeq 2000Homo sapiens12534-100mers-GM12878_186-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087866Illumina HiSeq 2000Homo sapiens12535-100mers-GM12878_187-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087867Illumina HiSeq 2000Homo sapiens12536-100mers-GM12878_188-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087868Illumina HiSeq 2000Homo sapiens12537-100mers-GM12878_189-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087869Illumina HiSeq 2000Homo sapiens12538-100mers-GM12878_190-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087870Illumina HiSeq 2000Homo sapiens12539-100mers-GM12878_191-TopHat-1.4.1-GENCODE-V13GM12878
GSE44618GSM1087871Illumina HiSeq 2000Homo sapiens12540-100mers-GM12878_192-TopHat-1.4.1-GENCODE-V13GM12878

Gene rank in GM12878