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Overall Design3 Single mouse ES cells, 7 single HEK293T cells, 8 diluted mouse ES total RNA samples and 19 mouse embryo samples are performed using SUPeR-seq to evaluate its ability to detect genes in single RNAs and to cover poly(A)- RNA species.
SummaryWe reported a new method of single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) to obtain single cell whole transcriptome analysis that covers both poly(A) plus and poly(A) minus RNA species. We built libraries of single mouse ESC , 10pg, 100pg and 1ng diluted mouse ES total RNA, single HEK293T cell and mouse early embryos before 4-cell stage. We analyzed these data using bioinformatics and find a good coverage of non-polyadenylated RNAs, circular RNA for example. to make a comparison,we also used mouse ES cells and HEK293T cells to build libraries using the protocol publish by F.Tang in 2009(sample name Tang2009_*). We also de novo assembled new genes in mouse ESCs and early embryo samples, further validated known and novel zygotic genes with a-Aminatine treated 2-cell embryos(sample name SUPeR-seq_a-AM_treated_2-cell*).
Dataset viewGSE53386

Samples in HEK293T

Displaying 1-10 of 10 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE53386GSM1290632Illumina HiSeq 2000Homo sapiensSUPeR-seq__HEK293T1HEK293T
GSE53386GSM1290633Illumina HiSeq 2000Homo sapiensSUPeR-seq__HEK293T2HEK293T
GSE53386GSM1290634Illumina HiSeq 2500Homo sapiensSUPeR-seq__HEK293T3HEK293T
GSE53386GSM1290635Illumina HiSeq 2500Homo sapiensSUPeR-seq__HEK293T4HEK293T
GSE53386GSM1290636Illumina HiSeq 2500Homo sapiensSUPeR-seq__HEK293T5HEK293T
GSE53386GSM1290637Illumina HiSeq 2500Homo sapiensSUPeR-seq__HEK293T6HEK293T
GSE53386GSM1290638Illumina HiSeq 2500Homo sapiensSUPeR-seq__HEK293T7HEK293T
GSE53386GSM1290639Illumina HiSeq 2500Homo sapiensTang2009-HEK293T1HEK293T
GSE53386GSM1290640Illumina HiSeq 2500Homo sapiensTang2009-HEK293T2HEK293T
GSE53386GSM1290641Illumina HiSeq 2500Homo sapiensTang2009-HEK293T3HEK293T

Gene rank in HEK293T