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  Human Skeletal Muscle Myoblasts (HSMM)

Overall DesignWe selected primary human myoblasts as a model system of cell differentiation to investigate whether ordering cells by progress revealed new regulators of the process. We sequenced RNA-Seq libraries from each of several hundred cells taken over a time-course of serum-induced differentiation.
SummarySingle-cell expression profiling by RNA-Seq promises to exploit cell-to-cell variation in gene expression to reveal regulatory circuitry governing cell differentiation and other biological processes. Here, we describe Monocle, a novel unsupervised algorithm for ordering cells by progress through differentiation that dramatically increases temporal resolution of expression measurements. This reordering unmasks switch-like changes in expression of key regulatory factors, reveals sequentially organized waves of gene regulation, and exposes regulators of cell differentiation. A functional screen confirms that a number of these regulators dramatically alter the efficiency of myoblast differentiation, demonstrating that single-cell expression analysis with Monocle can uncover new regulators even in well-studied systems.
Dataset viewGSE52529

Samples in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 1-10 of 372 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE52529GSM1268960Illumina HiSeq 2500Homo sapiensCell T0_CT_A01Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268961Illumina HiSeq 2500Homo sapiensCell T0_CT_A02Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268962Illumina HiSeq 2500Homo sapiensCell T0_CT_A03Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268963Illumina HiSeq 2500Homo sapiensCell T0_CT_A04Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268964Illumina HiSeq 2500Homo sapiensCell T0_CT_A05Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268965Illumina HiSeq 2500Homo sapiensCell T0_CT_A06Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268966Illumina HiSeq 2500Homo sapiensCell T0_CT_A07Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268967Illumina HiSeq 2500Homo sapiensCell T0_CT_A08Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268968Illumina HiSeq 2500Homo sapiensCell T0_CT_A09Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268969Illumina HiSeq 2500Homo sapiensCell T0_CT_A10Human Skeletal Muscle Myoblasts (HSMM)

Gene rank in Human Skeletal Muscle Myoblasts (HSMM)