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  Human Skeletal Muscle Myoblasts (HSMM)

Overall DesignWe selected primary human myoblasts as a model system of cell differentiation to investigate whether ordering cells by progress revealed new regulators of the process. We sequenced RNA-Seq libraries from each of several hundred cells taken over a time-course of serum-induced differentiation.
SummarySingle-cell expression profiling by RNA-Seq promises to exploit cell-to-cell variation in gene expression to reveal regulatory circuitry governing cell differentiation and other biological processes. Here, we describe Monocle, a novel unsupervised algorithm for ordering cells by progress through differentiation that dramatically increases temporal resolution of expression measurements. This reordering unmasks switch-like changes in expression of key regulatory factors, reveals sequentially organized waves of gene regulation, and exposes regulators of cell differentiation. A functional screen confirms that a number of these regulators dramatically alter the efficiency of myoblast differentiation, demonstrating that single-cell expression analysis with Monocle can uncover new regulators even in well-studied systems.
Dataset viewGSE52529

Samples in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 11-20 of 372 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE52529GSM1268970Illumina HiSeq 2500Homo sapiensCell T0_CT_A11Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268971Illumina HiSeq 2500Homo sapiensCell T0_CT_A12Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268972Illumina HiSeq 2500Homo sapiensCell T0_CT_B01Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268973Illumina HiSeq 2500Homo sapiensCell T0_CT_B02Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268974Illumina HiSeq 2500Homo sapiensCell T0_CT_B03Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268975Illumina HiSeq 2500Homo sapiensCell T0_CT_B04Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268976Illumina HiSeq 2500Homo sapiensCell T0_CT_B05Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268977Illumina HiSeq 2500Homo sapiensCell T0_CT_B06Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268978Illumina HiSeq 2500Homo sapiensCell T0_CT_B07Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1268979Illumina HiSeq 2500Homo sapiensCell T0_CT_B08Human Skeletal Muscle Myoblasts (HSMM)

Gene rank in Human Skeletal Muscle Myoblasts (HSMM)