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  Human Skeletal Muscle Myoblasts (HSMM)

Overall DesignWe selected primary human myoblasts as a model system of cell differentiation to investigate whether ordering cells by progress revealed new regulators of the process. We sequenced RNA-Seq libraries from each of several hundred cells taken over a time-course of serum-induced differentiation.
SummarySingle-cell expression profiling by RNA-Seq promises to exploit cell-to-cell variation in gene expression to reveal regulatory circuitry governing cell differentiation and other biological processes. Here, we describe Monocle, a novel unsupervised algorithm for ordering cells by progress through differentiation that dramatically increases temporal resolution of expression measurements. This reordering unmasks switch-like changes in expression of key regulatory factors, reveals sequentially organized waves of gene regulation, and exposes regulators of cell differentiation. A functional screen confirms that a number of these regulators dramatically alter the efficiency of myoblast differentiation, demonstrating that single-cell expression analysis with Monocle can uncover new regulators even in well-studied systems.
Dataset viewGSE52529
PMID24658644

Samples in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 21-30 of 372 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE52529GSM1269268Illumina HiSeq 2000Homo sapiensCell T72_CT_B12Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269269Illumina HiSeq 2000Homo sapiensCell T72_CT_C01Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269270Illumina HiSeq 2000Homo sapiensCell T72_CT_C02Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269271Illumina HiSeq 2000Homo sapiensCell T72_CT_C03Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269272Illumina HiSeq 2000Homo sapiensCell T72_CT_C04Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269273Illumina HiSeq 2000Homo sapiensCell T72_CT_C06Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269274Illumina HiSeq 2000Homo sapiensCell T72_CT_C07Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269275Illumina HiSeq 2000Homo sapiensCell T72_CT_C08Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269276Illumina HiSeq 2000Homo sapiensCell T72_CT_C09Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269277Illumina HiSeq 2000Homo sapiensCell T72_CT_C10Human Skeletal Muscle Myoblasts (HSMM)

Gene rank in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 41-50 of 19478 results.
Rank orderGene SymbolEnsembl ID
41RPS23ENSG00000186468
42RPL7ENSG00000147604
43RPLP0ENSG00000089157
44RPL19ENSG00000108298
45MT-ND2ENSG00000198763
46RPL5ENSG00000122406
47RPLP1ENSG00000137818
48RPL15ENSG00000174748
49PPIAENSG00000196262
50RPL35ENSG00000136942