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  Human Skeletal Muscle Myoblasts (HSMM)

Overall DesignWe selected primary human myoblasts as a model system of cell differentiation to investigate whether ordering cells by progress revealed new regulators of the process. We sequenced RNA-Seq libraries from each of several hundred cells taken over a time-course of serum-induced differentiation.
SummarySingle-cell expression profiling by RNA-Seq promises to exploit cell-to-cell variation in gene expression to reveal regulatory circuitry governing cell differentiation and other biological processes. Here, we describe Monocle, a novel unsupervised algorithm for ordering cells by progress through differentiation that dramatically increases temporal resolution of expression measurements. This reordering unmasks switch-like changes in expression of key regulatory factors, reveals sequentially organized waves of gene regulation, and exposes regulators of cell differentiation. A functional screen confirms that a number of these regulators dramatically alter the efficiency of myoblast differentiation, demonstrating that single-cell expression analysis with Monocle can uncover new regulators even in well-studied systems.
Dataset viewGSE52529
PMID24658644

Samples in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 41-50 of 372 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE52529GSM1269000Illumina HiSeq 2500Homo sapiensCell T0_CT_D05Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269001Illumina HiSeq 2500Homo sapiensCell T0_CT_D06Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269002Illumina HiSeq 2500Homo sapiensCell T0_CT_D07Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269003Illumina HiSeq 2500Homo sapiensCell T0_CT_D08Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269004Illumina HiSeq 2500Homo sapiensCell T0_CT_D09Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269005Illumina HiSeq 2500Homo sapiensCell T0_CT_D10Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269006Illumina HiSeq 2500Homo sapiensCell T0_CT_D11Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269007Illumina HiSeq 2500Homo sapiensCell T0_CT_D12Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269008Illumina HiSeq 2500Homo sapiensCell T0_CT_E01Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269009Illumina HiSeq 2500Homo sapiensCell T0_CT_E02Human Skeletal Muscle Myoblasts (HSMM)

Gene rank in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 19471-19478 of 19478 results.
Rank orderGene SymbolEnsembl ID
19471TTBK1ENSG00000146216
19472LINC01527ENSG00000224308
19473PSORS1C2ENSG00000204538
19474LINC01136ENSG00000233791
19475ALOX15BENSG00000179593
19476SNAP25ENSG00000132639
19477RAB6CENSG00000222014
19478KLHL32ENSG00000186231