Loading

Cell View

Cell Information

  Human Skeletal Muscle Myoblasts (HSMM)

Overall DesignWe selected primary human myoblasts as a model system of cell differentiation to investigate whether ordering cells by progress revealed new regulators of the process. We sequenced RNA-Seq libraries from each of several hundred cells taken over a time-course of serum-induced differentiation.
SummarySingle-cell expression profiling by RNA-Seq promises to exploit cell-to-cell variation in gene expression to reveal regulatory circuitry governing cell differentiation and other biological processes. Here, we describe Monocle, a novel unsupervised algorithm for ordering cells by progress through differentiation that dramatically increases temporal resolution of expression measurements. This reordering unmasks switch-like changes in expression of key regulatory factors, reveals sequentially organized waves of gene regulation, and exposes regulators of cell differentiation. A functional screen confirms that a number of these regulators dramatically alter the efficiency of myoblast differentiation, demonstrating that single-cell expression analysis with Monocle can uncover new regulators even in well-studied systems.
Dataset viewGSE52529
PMID24658644

Samples in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 1-10 of 372 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE52529GSM1269331Illumina HiSeq 2000Homo sapiensCell T72_CT_H12Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269330Illumina HiSeq 2000Homo sapiensCell T72_CT_H11Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269329Illumina HiSeq 2000Homo sapiensCell T72_CT_H10Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269328Illumina HiSeq 2000Homo sapiensCell T72_CT_H09Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269327Illumina HiSeq 2000Homo sapiensCell T72_CT_H08Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269326Illumina HiSeq 2000Homo sapiensCell T72_CT_H06Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269325Illumina HiSeq 2000Homo sapiensCell T72_CT_H05Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269324Illumina HiSeq 2000Homo sapiensCell T72_CT_H04Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269323Illumina HiSeq 2000Homo sapiensCell T72_CT_H03Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269322Illumina HiSeq 2000Homo sapiensCell T72_CT_H02Human Skeletal Muscle Myoblasts (HSMM)

Gene rank in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 31-40 of 19478 results.
Rank orderGene SymbolEnsembl ID
31B2MENSG00000166710
32RPS11ENSG00000142534
33RPS6ENSG00000137154
34RPS29ENSG00000213741
35RACK1ENSG00000204628
36FN1ENSG00000115414
37RPL27ENSG00000131469
38MALAT1ENSG00000251562
39RPL10ENSG00000147403
40RPS14ENSG00000164587