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  MCF10a human breast cancer cells

Overall DesignA microfluidic device that pairs sequence-barcoded mRNA capture beads with individual cells was used to barcode cDNA from individual cells which was then pre-amplified by in vitro transcription in a pool and converted into an Illumina RNA-Seq library. Libraries were generated from ~600 individual cells in parallel and extensive analysis was done on 396 cells from the U87 and MCF10a cell lines and from ~500 individual cells with extensive analysis on 247 cells from the U87 and WI-38 cell lines. Sequencing was done on the 3'-end of the transcript molecules. The first read contains cell-identifying barcodes that were present on the capture bead and the second read contains a unique molecular identifier (UMI) barcode, a lane-identifying barcode, and then the sequence of the transcript.
SummarySingle cell transcriptomics has emerged as a powerful approach to dissecting phenotypic heterogeneity in complex, unsynchronized cellular populations. However, many important biological questions demand quantitative analysis of large numbers of individual cells. Hence, new tools are urgently needed for efficient, inexpensive, and parallel manipulation of RNA from individual cells. We report a simple microfluidic platform for trapping single cell lysates in sealed, picoliter microwells capable of “printing” RNA on glass or capturing RNA on polymer beads. To demonstrate the utility of our system for single cell transcriptomics, we developed a highly scalable technology for genome-wide, single cell RNA-Seq. The current implementation of our device is pipette-operated, profiles hundreds of individual cells in parallel with library preparation costs of ~$0.10-$0.20/cell, and includes five lanes for simultaneous experiments. We anticipate that this system will ultimately serve as a general platform for large-scale single cell transcriptomics, compatible with both imaging and sequencing readouts.type = Expression profiling by high throughput sequencing
Dataset viewGSE66357
PMID26047807

Samples in MCF10a human breast cancer cells

Displaying 71-80 of 102 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE66357GSM1620686Illumina NextSeq 500Homo sapiensPS034_R2_4_724MCF10a human breast cancer cells
GSE66357GSM1620687Illumina NextSeq 500Homo sapiensPS034_R2_4_725MCF10a human breast cancer cells
GSE66357GSM1620688Illumina NextSeq 500Homo sapiensPS034_R2_4_75MCF10a human breast cancer cells
GSE66357GSM1620689Illumina NextSeq 500Homo sapiensPS034_R2_4_750MCF10a human breast cancer cells
GSE66357GSM1620690Illumina NextSeq 500Homo sapiensPS034_R2_4_76MCF10a human breast cancer cells
GSE66357GSM1620691Illumina NextSeq 500Homo sapiensPS034_R2_4_761MCF10a human breast cancer cells
GSE66357GSM1620692Illumina NextSeq 500Homo sapiensPS034_R2_4_763MCF10a human breast cancer cells
GSE66357GSM1620693Illumina NextSeq 500Homo sapiensPS034_R2_4_764MCF10a human breast cancer cells
GSE66357GSM1620694Illumina NextSeq 500Homo sapiensPS034_R2_4_765MCF10a human breast cancer cells
GSE66357GSM1620695Illumina NextSeq 500Homo sapiensPS034_R2_4_779MCF10a human breast cancer cells

Gene rank in MCF10a human breast cancer cells

Displaying 18961-18970 of 18975 results.
Rank orderGene SymbolEnsembl ID
18961ZNF823ENSG00000197933
18962ZNF837ENSG00000152475
18963ZNF846ENSG00000196605
18964ZNF852ENSG00000178917
18965ZNF891ENSG00000214029
18966ZNRF3ENSG00000183579
18967ZP3ENSG00000188372
18968ZRANB2-AS2ENSG00000229956
18969ZRSR1ENSG00000212643
18970ZSCAN12ENSG00000158691