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  PDX primary renal cell carcinoma

Overall DesignIn order to identify successful clonal propagation from patient to PDX samples and understand pathogenesis from primary to metastatic RCC, we performed whole-exome sequencing (WES, n=4) and matched aCGH (n=4) on bulk tumor samples. And we utilized single-cell RNA sequencing (scRNA-seq) to model and dissect functional heterogeneity acroass primary and metastatic RCC tumors. We checked whether of capturing live one cell, not more cells, in microfluidics by fluorescent microscopic observation. To construct RNA sequencing libraries, we performed further quality controls including adequate quantities and qualities of amplified transcriptomes respectively from single cells. Tumor cells from the parental mRCC (n=34), PDX-mRCC (n=36) and PDX-pRCC (n=46) were finally analyzed in this study after filtering out poor quality cells.
SummaryClear cell renal cell carcinoma (ccRCC) initiated from the renal epithelium is the most prevalent histological type of adult kidney cancers. Dissecting intratumoral heterogeneity (ITH) of ccRCC has leveraged to extend our knowledge on how primary tumors harboring driver mutations evolve and spread to other sites. The cellular fractions within and across the primary (pRCC) and metastatic RCC (mRCC) are heterogeneous in both their genetic and biological features determining the variability in clinical aggressiveness and sensitivity to the therapy. To achieve sustainable therapeutic benefit with targeted agents in mRCC, the effective target should focus on signaling pathways that are related to driver mutations occurred early in the clonal evolution of the disease and thus should be common to primary tumor and metastatic sites. Considering that extensive genetic heterogeneity may result in drug response variability among patients and treatment resistance, the tailored strategies for metastatic RCC is urgently needed. Here, we analyze single-cell RNA-seq (scRNA-seq) data from a matched primary RCC (pRCC) and lung metastasis (mRCC) to dissect ITH at the highest resolution to date with the objective of discovering the better therapeutic regimen.
Dataset viewGSE73121

Samples in PDX primary renal cell carcinoma

Displaying 11-20 of 47 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE73121GSM1887263Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_29PDX primary renal cell carcinoma
GSE73121GSM1887264Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_34PDX primary renal cell carcinoma
GSE73121GSM1887265Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_35PDX primary renal cell carcinoma
GSE73121GSM1887266Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_36PDX primary renal cell carcinoma
GSE73121GSM1887267Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_37PDX primary renal cell carcinoma
GSE73121GSM1887268Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_46PDX primary renal cell carcinoma
GSE73121GSM1887269Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_49PDX primary renal cell carcinoma
GSE73121GSM1887270Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_50PDX primary renal cell carcinoma
GSE73121GSM1887271Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_51PDX primary renal cell carcinoma
GSE73121GSM1887272Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_52PDX primary renal cell carcinoma

Gene rank in PDX primary renal cell carcinoma

Displaying 11-20 of 18370 results.
Rank orderGene SymbolEnsembl ID