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  Primordial Germ Cells[male 11 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 11 week gestation]

Displaying 11-20 of 27 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677548Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo1_sc11Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677549Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo1_sc12Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677550Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc1Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677551Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc2Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677552Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc3Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677553Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc4Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677554Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc5Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677555Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc6Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677556Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc7Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677557Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc8Primordial Germ Cells[male 11 week gestation]

Gene rank in Primordial Germ Cells[male 11 week gestation]

Displaying 11-20 of 21483 results.
Rank orderGene SymbolEnsembl ID
11RPS3AENSG00000145425
12RPL26ENSG00000161970
13RPL5ENSG00000122406
14RPL11ENSG00000142676
15RPL19ENSG00000108298
16RPLP1ENSG00000137818
17RPS13ENSG00000110700
18GNB2L1
19RPS8ENSG00000142937
20EEF1GENSG00000254772