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  Primordial Germ Cells[male 11 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 11 week gestation]

Displaying 21-27 of 27 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677558Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc9Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677559Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc10Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677560Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc11Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677561Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc12Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677562Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc13Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677563Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc14Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677564Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc15Primordial Germ Cells[male 11 week gestation]

Gene rank in Primordial Germ Cells[male 11 week gestation]

Displaying 61-70 of 21483 results.
Rank orderGene SymbolEnsembl ID
61HSPA8ENSG00000109971
62EEF1B2ENSG00000114942
63RPL18ENSG00000063177
64RPL35AENSG00000182899
65NPM1ENSG00000181163
66RPL23AENSG00000198242
67RPS27AENSG00000143947
68HSP90AB1ENSG00000096384
69RPL23ENSG00000125691
70RPL31ENSG00000071082