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  Primordial Germ Cells[male 19 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 19 week gestation]

Displaying 51-57 of 57 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677615Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc20Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677616Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc21Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677617Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc22Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677618Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc23Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677619Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc24Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677620Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc25Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677621Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc26Primordial Germ Cells[male 19 week gestation]

Gene rank in Primordial Germ Cells[male 19 week gestation]

Displaying 81-90 of 21483 results.
Rank orderGene SymbolEnsembl ID
81RPL27ENSG00000131469
82PTGES3ENSG00000110958
83BTF3ENSG00000145741
84RPL18ENSG00000063177
85RPL35ENSG00000136942
86RPL15ENSG00000174748
87C14orf166ENSG00000087302
88CCT2ENSG00000166226
89TCP1ENSG00000120438
90RPS9ENSG00000170889