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  Primordial Germ Cells[male 7 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 7 week gestation]

Displaying 11-20 of 40 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677489Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc11Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677490Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc12Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677491Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc13Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677492Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc14Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677493Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc15Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677494Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc16Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677495Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo2_sc1Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677496Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo2_sc2Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677497Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo2_sc3Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677498Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo2_sc4Primordial Germ Cells[male 7 week gestation]

Gene rank in Primordial Germ Cells[male 7 week gestation]

Displaying 21481-21483 of 21483 results.
Rank orderGene SymbolEnsembl ID
21481ZSCAN4ENSG00000180532
21482ZSCAN5BENSG00000197213
21483ZSWIM2ENSG00000163012