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  Primordial Germ Cells[male 7 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 7 week gestation]

Displaying 31-40 of 40 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677509Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc5Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677510Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc6Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677511Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc7Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677512Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc8Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677513Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc9Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677514Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc10Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677515Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc11Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677516Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc12Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677517Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo3_sc13Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677518Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc1Primordial Germ Cells[male 7 week gestation]

Gene rank in Primordial Germ Cells[male 7 week gestation]

Displaying 41-50 of 21483 results.
Rank orderGene SymbolEnsembl ID
41POU5F1ENSG00000204531
42RPL24ENSG00000114391
43RPL37AENSG00000197756
44RPS15AENSG00000134419
45RPS25ENSG00000118181
46RPL30ENSG00000156482
47HNRNPA1ENSG00000135486
48ACTBENSG00000075624
49EEF1B2ENSG00000114942
50RPL23ENSG00000125691