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  Somatic Cells[female 4 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Somatic Cells[female 4 week gestation]

Displaying 1-6 of 6 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677754Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc1Somatic Cells[female 4 week gestation]
GSE63818GSM1677755Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc2Somatic Cells[female 4 week gestation]
GSE63818GSM1677756Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc3Somatic Cells[female 4 week gestation]
GSE63818GSM1677757Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc4Somatic Cells[female 4 week gestation]
GSE63818GSM1677758Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc5Somatic Cells[female 4 week gestation]
GSE63818GSM1677759Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc6Somatic Cells[female 4 week gestation]

Gene rank in Somatic Cells[female 4 week gestation]

Displaying 51-60 of 21483 results.
Rank orderGene SymbolEnsembl ID
51HSP90AB1ENSG00000096384
52HNRNPA1ENSG00000135486
53RPS9ENSG00000170889
54RPL21ENSG00000122026
55HSP90AA1ENSG00000080824
56HSPA8ENSG00000109971
57RPL31ENSG00000071082
58MDKENSG00000110492
59RPS15AENSG00000134419
60ENO1ENSG00000074800