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  Somatic Cells[male 7 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Somatic Cells[male 7 week gestation]

Displaying 11-20 of 26 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677716Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo1_sc11Somatic Cells[male 7 week gestation]
GSE63818GSM1677717Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo1_sc12Somatic Cells[male 7 week gestation]
GSE63818GSM1677718Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo1_sc13Somatic Cells[male 7 week gestation]
GSE63818GSM1677719Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc1Somatic Cells[male 7 week gestation]
GSE63818GSM1677720Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc2Somatic Cells[male 7 week gestation]
GSE63818GSM1677721Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc3Somatic Cells[male 7 week gestation]
GSE63818GSM1677722Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc4Somatic Cells[male 7 week gestation]
GSE63818GSM1677723Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc5Somatic Cells[male 7 week gestation]
GSE63818GSM1677724Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc6Somatic Cells[male 7 week gestation]
GSE63818GSM1677725Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo2_sc7Somatic Cells[male 7 week gestation]

Gene rank in Somatic Cells[male 7 week gestation]

Displaying 41-50 of 21483 results.
Rank orderGene SymbolEnsembl ID
41BEX1ENSG00000133169
42RPL12ENSG00000197958
43UBBENSG00000170315
44RPS14ENSG00000164587
45RPL35AENSG00000182899
46RPS24ENSG00000138326
47RPL24ENSG00000114391
48RPL23ENSG00000125691
49RPS25ENSG00000118181
50SERPINF1ENSG00000132386