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  Somatic Cells[male 7 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Somatic Cells[male 7 week gestation]

Displaying 21-26 of 26 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677726Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo3_sc1Somatic Cells[male 7 week gestation]
GSE63818GSM1677727Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo3_sc2Somatic Cells[male 7 week gestation]
GSE63818GSM1677728Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo3_sc3Somatic Cells[male 7 week gestation]
GSE63818GSM1677729Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo3_sc4Somatic Cells[male 7 week gestation]
GSE63818GSM1677730Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo3_sc5Somatic Cells[male 7 week gestation]
GSE63818GSM1677731Illumina HiSeq 2500Homo sapiensM_Soma_7W_embryo3_sc6Somatic Cells[male 7 week gestation]

Gene rank in Somatic Cells[male 7 week gestation]

Displaying 81-90 of 21483 results.
Rank orderGene SymbolEnsembl ID
81RPL15ENSG00000174748
82RPL18ENSG00000063177
83RPS15ENSG00000115268
84RPL29ENSG00000162244
85RPL27ENSG00000131469
86RPL35ENSG00000136942
87COL1A2ENSG00000164692
88EEF1B2ENSG00000114942
89RPL23AENSG00000198242
90HSP90AB1ENSG00000096384