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  U87 human glioma cells

Overall DesignA microfluidic device that pairs sequence-barcoded mRNA capture beads with individual cells was used to barcode cDNA from individual cells which was then pre-amplified by in vitro transcription in a pool and converted into an Illumina RNA-Seq library. Libraries were generated from ~600 individual cells in parallel and extensive analysis was done on 396 cells from the U87 and MCF10a cell lines and from ~500 individual cells with extensive analysis on 247 cells from the U87 and WI-38 cell lines. Sequencing was done on the 3'-end of the transcript molecules. The first read contains cell-identifying barcodes that were present on the capture bead and the second read contains a unique molecular identifier (UMI) barcode, a lane-identifying barcode, and then the sequence of the transcript.
SummarySingle cell transcriptomics has emerged as a powerful approach to dissecting phenotypic heterogeneity in complex, unsynchronized cellular populations. However, many important biological questions demand quantitative analysis of large numbers of individual cells. Hence, new tools are urgently needed for efficient, inexpensive, and parallel manipulation of RNA from individual cells. We report a simple microfluidic platform for trapping single cell lysates in sealed, picoliter microwells capable of “printing” RNA on glass or capturing RNA on polymer beads. To demonstrate the utility of our system for single cell transcriptomics, we developed a highly scalable technology for genome-wide, single cell RNA-Seq. The current implementation of our device is pipette-operated, profiles hundreds of individual cells in parallel with library preparation costs of ~$0.10-$0.20/cell, and includes five lanes for simultaneous experiments. We anticipate that this system will ultimately serve as a general platform for large-scale single cell transcriptomics, compatible with both imaging and sequencing readouts.type = Expression profiling by high throughput sequencing
Dataset viewGSE66357
PMID26047807

Samples in U87 human glioma cells

Displaying 61-70 of 138 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE66357GSM1620407Illumina NextSeq 500Homo sapiensPS034_R2_0_927U87 human glioma cells
GSE66357GSM1620408Illumina NextSeq 500Homo sapiensPS034_R2_0_942U87 human glioma cells
GSE66357GSM1620409Illumina NextSeq 500Homo sapiensPS034_R2_0_99U87 human glioma cells
GSE66357GSM1683185Illumina NextSeq 500Homo sapiensPS034_R2_0_16U87 human glioma cells
GSE66357GSM1683186Illumina NextSeq 500Homo sapiensPS034_R2_0_388U87 human glioma cells
GSE66357GSM1683187Illumina NextSeq 500Homo sapiensPS034_R2_0_891U87 human glioma cells
GSE66357GSM1683211Illumina NextSeq 500Homo sapiensPS041-2_R2_0_10U87 human glioma cells
GSE66357GSM1683212Illumina NextSeq 500Homo sapiensPS041-2_R2_0_105U87 human glioma cells
GSE66357GSM1683213Illumina NextSeq 500Homo sapiensPS041-2_R2_0_116U87 human glioma cells
GSE66357GSM1683214Illumina NextSeq 500Homo sapiensPS041-2_R2_0_132U87 human glioma cells

Gene rank in U87 human glioma cells

Displaying 71-80 of 18975 results.
Rank orderGene SymbolEnsembl ID
71__ambiguous[NME1-NME
72RPL10ENSG00000147403
73CCNIENSG00000118816
74RPS28ENSG00000233927
75RPL34ENSG00000109475
76RPS11ENSG00000142534
77RPS4XENSG00000198034
78RPL27AENSG00000166441
79RPL7AENSG00000148303
80RPL31ENSG00000071082