Loading

Cell View

Cell Information

  cultured embryonic stem cells[DCX+][12]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX+][12]

Displaying 231-240 of 263 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2312078Illumina HiSeq 2500Homo sapiens12Dp1a_D06cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312073Illumina HiSeq 2500Homo sapiens12Dp1a_D04cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312071Illumina HiSeq 2500Homo sapiens12Dp1a_D03cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312063Illumina HiSeq 2500Homo sapiens12Dp1a_D02cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312072Illumina HiSeq 2500Homo sapiens12Dp1a_D01cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312042Illumina HiSeq 2500Homo sapiens12Dp1a_C10cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312075Illumina HiSeq 2500Homo sapiens12Dp1a_C09cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312050Illumina HiSeq 2500Homo sapiens12Dp1a_C07cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312051Illumina HiSeq 2500Homo sapiens12Dp1a_C06cultured embryonic stem cells[DCX+][12]
GSE86977GSM2312079Illumina HiSeq 2500Homo sapiens12Dp1a_C05cultured embryonic stem cells[DCX+][12]

Gene rank in cultured embryonic stem cells[DCX+][12]

Displaying 23551-23560 of 23636 results.
Rank orderGene SymbolEnsembl ID
23551UGT2B17ENSG00000197888
23552UGT2B28ENSG00000135226
23553ULBP2ENSG00000131015
23554ULBP3ENSG00000131019
23555ULK4P2ENSG00000260128
23556UNQ6975
23557UPK1A-AS1ENSG00000226510
23558UPK3AENSG00000100373
23559USP17L10ENSG00000231396
23560USP17L11ENSG00000233136