Loading

Cell View

Cell Information

  cultured embryonic stem cells[DCX+][54]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX+][54]

Displaying 451-460 of 495 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2313926Illumina HiSeq 2500Homo sapiens54Dp5a_A07cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313927Illumina HiSeq 2500Homo sapiens54Dp4a_D10cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313928Illumina HiSeq 2500Homo sapiens54Dp6a_C05cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313929Illumina HiSeq 2500Homo sapiens54Dp2b_D07cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313930Illumina HiSeq 2500Homo sapiens54Dp2b_G04cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314186Illumina HiSeq 2500Homo sapiens54Dp7b_D04cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313931Illumina HiSeq 2500Homo sapiens54Dp4a_C01cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313932Illumina HiSeq 2500Homo sapiens54Dp5b_D02cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313933Illumina HiSeq 2500Homo sapiens54Dp5b_C06cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313934Illumina HiSeq 2500Homo sapiens54Dp5b_C09cultured embryonic stem cells[DCX+][54]

Gene rank in cultured embryonic stem cells[DCX+][54]

Displaying 71-80 of 23636 results.
Rank orderGene SymbolEnsembl ID
71RPLP1ENSG00000137818
72H3F3BENSG00000132475
73RPL9ENSG00000163682
74HSPA8ENSG00000109971
75PTPRSENSG00000105426
76ENO1ENSG00000074800
77RPL41ENSG00000229117
78RPS4XENSG00000198034
79RTN4ENSG00000115310
80RPS14ENSG00000164587