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  cultured embryonic stem cells[DCX-][19]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX-][19]

Displaying 41-50 of 110 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2312340Illumina HiSeq 2500Homo sapiens19Dn2b_C06cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312341Illumina HiSeq 2500Homo sapiens19Dn2b_C05cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312342Illumina HiSeq 2500Homo sapiens19Dn2b_A06cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312343Illumina HiSeq 2500Homo sapiens19Dn2b_B06cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312344Illumina HiSeq 2500Homo sapiens19Dn2b_B05cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312345Illumina HiSeq 2500Homo sapiens19Dn2b_D07cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312346Illumina HiSeq 2500Homo sapiens19Dn2b_D06cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312347Illumina HiSeq 2500Homo sapiens19Dn2b_A05cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312348Illumina HiSeq 2500Homo sapiens19Dn2b_C04cultured embryonic stem cells[DCX-][19]
GSE86977GSM2312349Illumina HiSeq 2500Homo sapiens19Dn2b_D05cultured embryonic stem cells[DCX-][19]

Gene rank in cultured embryonic stem cells[DCX-][19]

Displaying 23601-23610 of 23636 results.
Rank orderGene SymbolEnsembl ID
23601WFDC6ENSG00000243543
23602WFDC8ENSG00000158901
23603WFIKKN2ENSG00000173714
23604WNT10BENSG00000169884
23605WNT16ENSG00000002745
23606WNT8BENSG00000075290
23607WNT9AENSG00000143816
23608WWTR1-AS1ENSG00000241313
23609XAGE1BENSG00000204382
23610XAGE1D