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  cultured embryonic stem cells[DCX-][26]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX-][26]

Displaying 31-40 of 85 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2313062Illumina HiSeq 2500Homo sapiens26Dn2_B06cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313063Illumina HiSeq 2500Homo sapiens26Dn2_B05cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313064Illumina HiSeq 2500Homo sapiens26Dn2_D07cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313065Illumina HiSeq 2500Homo sapiens26Dn2_D06cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313066Illumina HiSeq 2500Homo sapiens26Dn2_A05cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313067Illumina HiSeq 2500Homo sapiens26Dn2_B04cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313068Illumina HiSeq 2500Homo sapiens26Dn2_A04cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313069Illumina HiSeq 2500Homo sapiens26Dn2_D04cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313070Illumina HiSeq 2500Homo sapiens26Dn2_D03cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313071Illumina HiSeq 2500Homo sapiens26Dn2_B03cultured embryonic stem cells[DCX-][26]

Gene rank in cultured embryonic stem cells[DCX-][26]

Displaying 61-70 of 23636 results.
Rank orderGene SymbolEnsembl ID
61RPS7ENSG00000171863
62RPS14ENSG00000164587
63RPL4ENSG00000174444
64RPL3ENSG00000100316
65RPL8ENSG00000161016
66RPS4XENSG00000198034
67RPL34ENSG00000109475
68RPS10ENSG00000124614
69RPL23ENSG00000125691
70EEF1GENSG00000254772