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  cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis

Overall Design26 Llive and 19 Fixed cultured hESCs were prepared and sequenced using both FRISCR and TritonX-100 Lysis as proof of principal for FRSCR.
SummaryThe human neocortex is created from diverse progenitors that are intermixed with multiple cell types in the prenatal germinal zones. These progenitors have been difficult to profile with unbiased transcriptomics since progenitors-particularly radial glia (RG)-are rare cell types, defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed a method called FRSCR for transcriptome profiling of individual fixed, stained, and sorted cells. After validation of FRSCR with human embryonic stem cells, we profiled primary human RG that constitute only 1% of the mid-gestation cortex. These data showed that RG could be classified into ventricle zone-enriched RG (vRG) that expressed ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that expressed HOPX. Our study identified the first markers and molecular profiles of vRG and oRG cells, and provides an essential step for understanding molecular networks that control the development and lineage of human neocortical progenitors. Furthermore, FRSCR allows targeted single-cell transcriptomic profiling of many tissues that currently lack live-cell markers.
Dataset viewGSE71858
PMID26524239

Samples in cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis

Displaying 1-7 of 7 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE71858GSM1846968Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457350-6]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis
GSE71858GSM1846969Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457350-7]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis
GSE71858GSM1846970Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457350-8]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis
GSE71858GSM1846971Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457350-9]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis
GSE71858GSM1846983Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457351-20]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis
GSE71858GSM1846984Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457351-21]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis
GSE71858GSM1846985Illumina HiSeq 2500Homo sapiensFixed_RLT [US-1457351-22]cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis

Gene rank in cultured embryonic stem cells (H1) DAPI+ Fixed cells Triton X Lysis

Displaying 31-40 of 17315 results.
Rank orderGene SymbolEnsembl ID
31RPL23AENSG00000198242
32RPS3ENSG00000149273
33RPL8ENSG00000161016
34C14orf156
35RPS15ENSG00000115268
36RPL35AENSG00000182899
37RPL21ENSG00000122026
38RPL27ENSG00000131469
39PFN1ENSG00000108518
40AD1