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  d4 AG+ cells

Overall DesignRNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
SummaryMechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
Dataset viewGSE67259
PMID26189426

Samples in d4 AG+ cells

Displaying 1-2 of 2 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE67259GSM1643175AB 5500xl Genetic AnalyzerHomo sapiensd4_wt1d4 AG+ cells
GSE67259GSM1643176AB 5500xl Genetic AnalyzerHomo sapiensd4_wt2d4 AG+ cells

Gene rank in d4 AG+ cells

Displaying 1-10 of 20748 results.
Rank orderGene SymbolEnsembl ID
1EEF1A1ENSG00000156508
2RPS24ENSG00000138326
3RPL9ENSG00000163682
4LOC100505829
5RPLP0ENSG00000089157
6RPS27AENSG00000143947
7RPS3AENSG00000145425
8RPL7AENSG00000148303
9RPL13AENSG00000142541
10RPS7ENSG00000171863