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  glioblastoma [Neu]

Overall DesignOperative specimens from five glioblastoma patients (MGH26, MGH28, MGH29, MGH30, MGH31) were acutely dissociated, depleted for CD45+ inflammatory cells and then sorted as single cells (576 samples). Population controls for each tumor were isolated by sorting 2000-10000 cells and processed in parallel (5 population control samples). Single cells from two established cell lines, GBM6 and GBM8, were also sorted as single cells (192 samples).
SummaryWe report transcriptomes from 430 single glioblastoma cells isolated from 5 individual tumors and 102 single cells from gliomasphere cells lines generated using SMART-seq. In addition, we report population RNA-seq from the five tumors as well as RNA-seq from cell lines derived from 3 tumors (MGH26, MGH28, MGH31) cultured under serum free (GSC) and differentiated (DGC) conditions. This dataset highlights intratumoral heterogeneity with regards to the expression of de novo derived transcriptional modules and established subtype classifiers.
Dataset viewGSE57872
PMID24925914

Samples in glioblastoma [Neu]

Displaying 1-10 of 24 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE57872GSM1395440Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26_D06glioblastoma [Neu]
GSE57872GSM1395689Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_A03glioblastoma [Neu]
GSE57872GSM1395704Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_B06glioblastoma [Neu]
GSE57872GSM1395713Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_C03glioblastoma [Neu]
GSE57872GSM1395718Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_C08glioblastoma [Neu]
GSE57872GSM1395725Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_D03glioblastoma [Neu]
GSE57872GSM1395764Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_G06glioblastoma [Neu]
GSE57872GSM1395771Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_H01glioblastoma [Neu]
GSE57872GSM1395772Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_H02glioblastoma [Neu]
GSE57872GSM1395774Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_H04glioblastoma [Neu]

Gene rank in glioblastoma [Neu]

Displaying 11-20 of 5942 results.
Rank orderGene SymbolEnsembl ID
11TPI1ENSG00000111669
12TMBIM6ENSG00000139644
13ENO1ENSG00000074800
14UBCENSG00000150991
15C6orf62ENSG00000112308
16B2MENSG00000166710
17DPYSL2ENSG00000092964
18PSAPENSG00000197746
19PCSK5ENSG00000099139
20LDHAENSG00000134333