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  glioblastoma [None]

Overall DesignOperative specimens from five glioblastoma patients (MGH26, MGH28, MGH29, MGH30, MGH31) were acutely dissociated, depleted for CD45+ inflammatory cells and then sorted as single cells (576 samples). Population controls for each tumor were isolated by sorting 2000-10000 cells and processed in parallel (5 population control samples). Single cells from two established cell lines, GBM6 and GBM8, were also sorted as single cells (192 samples).
SummaryWe report transcriptomes from 430 single glioblastoma cells isolated from 5 individual tumors and 102 single cells from gliomasphere cells lines generated using SMART-seq. In addition, we report population RNA-seq from the five tumors as well as RNA-seq from cell lines derived from 3 tumors (MGH26, MGH28, MGH31) cultured under serum free (GSC) and differentiated (DGC) conditions. This dataset highlights intratumoral heterogeneity with regards to the expression of de novo derived transcriptional modules and established subtype classifiers.
Dataset viewGSE57872
PMID24925914

Samples in glioblastoma [None]

Displaying 1-10 of 120 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE57872GSM1395509Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_B03glioblastoma [None]
GSE57872GSM1395519Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_C01glioblastoma [None]
GSE57872GSM1395781Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH29_H11glioblastoma [None]
GSE57872GSM1395790Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_A08glioblastoma [None]
GSE57872GSM1395793Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_A11glioblastoma [None]
GSE57872GSM1395794Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_A12glioblastoma [None]
GSE57872GSM1395796Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_B02glioblastoma [None]
GSE57872GSM1395542Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_D12glioblastoma [None]
GSE57872GSM1395803Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_B09glioblastoma [None]
GSE57872GSM1395806Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_B12glioblastoma [None]

Gene rank in glioblastoma [None]