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  hES hiPS-derived neuron

Overall DesignPatch-Seq of 20 hESC/hiPSC-derived neurons.
SummaryThe mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion are generated. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single-cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here, we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single-cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
Dataset viewGSE77564
PMID26883038

Samples in hES hiPS-derived neuron

Displaying 11-20 of 20 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE77564GSM2054562Illumina HiSeq 2500Homo sapiensSingle neuron 19hES hiPS-derived neuron
GSE77564GSM2054545Illumina HiSeq 2500Homo sapiensSingle neuron 2hES hiPS-derived neuron
GSE77564GSM2054563Illumina HiSeq 2500Homo sapiensSingle neuron 20hES hiPS-derived neuron
GSE77564GSM2054546Illumina HiSeq 2500Homo sapiensSingle neuron 3hES hiPS-derived neuron
GSE77564GSM2054547Illumina HiSeq 2500Homo sapiensSingle neuron 4hES hiPS-derived neuron
GSE77564GSM2054548Illumina HiSeq 2500Homo sapiensSingle neuron 5hES hiPS-derived neuron
GSE77564GSM2054549Illumina HiSeq 2500Homo sapiensSingle neuron 6hES hiPS-derived neuron
GSE77564GSM2054550Illumina HiSeq 2500Homo sapiensSingle neuron 7hES hiPS-derived neuron
GSE77564GSM2054551Illumina HiSeq 2500Homo sapiensSingle neuron 8hES hiPS-derived neuron
GSE77564GSM2054552Illumina HiSeq 2500Homo sapiensSingle neuron 9hES hiPS-derived neuron

Gene rank in hES hiPS-derived neuron

Displaying 11-20 of 19234 results.
Rank orderGene SymbolEnsembl ID
11FTLENSG00000087086
12HIST1H4CENSG00000197061
13NDUFB2ENSG00000090266
14RPL41ENSG00000229117
15MIR1273AENSG00000221753
16RPS19ENSG00000105372
17RPL23ENSG00000125691
18MTRNR2L10ENSG00000256045
19GAPDHENSG00000111640
20RPS14ENSG00000164587