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  human K562 cells

Overall DesignA total of 8 single cell data sets are submitted: 3 for mouse embryonic stem (ES) cells (1 biological replicate, 2 technical replicates); 3 samples following LIF withdrawal (days 2,4, 7); one pure RNA data set (from human lymphoblast K562 cells); and one sample of single K562 cells.
SummaryRecently, RNA sequencing has achieved single cell resolution, but what is limiting is an effective way to routinely isolate and process large numbers of individual cells for in-depth sequencing, and to do so quantitatively. We have developed a droplet-microfluidic approach for parallel barcoding thousands of individual cells for subsequent RNA profiling by next-generation sequencing. This high-throughput method shows a surprisingly low noise profile and is readily adaptable to other sequencing-based assays. Using this technique, we analyzed mouse embryonic stem cells, revealing in detail the population structure and the heterogeneous onset of differentiation after LIF withdrawal. The reproducibility and low noise of this high-throughput single cell data allowed us to deconstruct cell populations and infer gene expression relationships.
Dataset viewGSE65525
PMID26000487

Samples in human K562 cells

Displaying 11-20 of 239 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE65525GSM1599500.114Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.115Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.116Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.117Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.118Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.119Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.120Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.121Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.122Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.123Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells

Gene rank in human K562 cells

Displaying 24071-24080 of 24121 results.
Rank orderGene SymbolEnsembl ID
24071DEARENSG00000270751
24072ECRP
24073MIR1289-2ENSG00000221287
24074MIR302FENSG00000283218
24075MIR3612ENSG00000265635
24076MIR6499ENSG00000276622
24077MIR6796ENSG00000275652
24078RNU5F-1ENSG00000199377
24079RNU6-81PENSG00000202478
24080SNORD114-12ENSG00000202270