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  human K562 cells

Overall DesignA total of 8 single cell data sets are submitted: 3 for mouse embryonic stem (ES) cells (1 biological replicate, 2 technical replicates); 3 samples following LIF withdrawal (days 2,4, 7); one pure RNA data set (from human lymphoblast K562 cells); and one sample of single K562 cells.
SummaryRecently, RNA sequencing has achieved single cell resolution, but what is limiting is an effective way to routinely isolate and process large numbers of individual cells for in-depth sequencing, and to do so quantitatively. We have developed a droplet-microfluidic approach for parallel barcoding thousands of individual cells for subsequent RNA profiling by next-generation sequencing. This high-throughput method shows a surprisingly low noise profile and is readily adaptable to other sequencing-based assays. Using this technique, we analyzed mouse embryonic stem cells, revealing in detail the population structure and the heterogeneous onset of differentiation after LIF withdrawal. The reproducibility and low noise of this high-throughput single cell data allowed us to deconstruct cell populations and infer gene expression relationships.
Dataset viewGSE65525
PMID26000487

Samples in human K562 cells

Displaying 171-180 of 239 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE65525GSM1599500.35Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.36Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.37Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.38Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.39Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.40Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.41Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.42Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.43Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.44Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells

Gene rank in human K562 cells

Displaying 24051-24060 of 24121 results.
Rank orderGene SymbolEnsembl ID
24051MIR451BENSG00000283364
24052MIR937
24053MOB4ENSG00000115540
24054TPPP2ENSG00000179636
24055KRTAP23-1ENSG00000186980
24056MIR3976ENSG00000272380
24057MIR4757ENSG00000266738
24058MIR6847
24059MEIG1ENSG00000197889
24060MIR4515ENSG00000263643