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  human K562 cells

Overall DesignA total of 8 single cell data sets are submitted: 3 for mouse embryonic stem (ES) cells (1 biological replicate, 2 technical replicates); 3 samples following LIF withdrawal (days 2,4, 7); one pure RNA data set (from human lymphoblast K562 cells); and one sample of single K562 cells.
SummaryRecently, RNA sequencing has achieved single cell resolution, but what is limiting is an effective way to routinely isolate and process large numbers of individual cells for in-depth sequencing, and to do so quantitatively. We have developed a droplet-microfluidic approach for parallel barcoding thousands of individual cells for subsequent RNA profiling by next-generation sequencing. This high-throughput method shows a surprisingly low noise profile and is readily adaptable to other sequencing-based assays. Using this technique, we analyzed mouse embryonic stem cells, revealing in detail the population structure and the heterogeneous onset of differentiation after LIF withdrawal. The reproducibility and low noise of this high-throughput single cell data allowed us to deconstruct cell populations and infer gene expression relationships.
Dataset viewGSE65525
PMID26000487

Samples in human K562 cells

Displaying 31-40 of 239 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE65525GSM1599500.134Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.135Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.136Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.137Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.138Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.139Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.140Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.141Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.142Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.143Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells

Gene rank in human K562 cells

Displaying 71-80 of 24121 results.
Rank orderGene SymbolEnsembl ID
71RPL27ENSG00000131469
72RPL5ENSG00000122406
73HSPA8ENSG00000109971
74RPS27AENSG00000143947
75SNHG3ENSG00000242125
76RPL10AENSG00000198755
77RPL37ENSG00000145592
78RPS10ENSG00000124614
79RPL37AENSG00000197756
80HIST1H1CENSG00000187837