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  iPSC 409B2[35 days]

Overall Design734 single-cell transcriptomes from human fetal neocortex or human cerebral organoids from multiple time points were analyzed in this study. All single cell samples were processed on the microfluidic Fluidigm C1 platform and contain 92 external RNA spike-ins. Fetal neocortex data were generated at 12 weeks post conception (chip 1: 81 cells; chip 2: 83 cells) and 13 weeks post conception (62 cells). Cerebral organoid data were generated from dissociated whole organoids derived from induced pluripotent stem cell line 409B2 (iPSC 409B2) at 33 days (40 cells), 35 days (68 cells), 37 days (71 cells), 41 days (74 cells), and 65 days (80 cells) after the start of embryoid body culture. Cerebral organoid data were also generated from microdissected cortical-like regions from H9 embryonic stem cell derived organoids at 53 days (region 1, 48 cells; region 2, 48 cells) or from iPSC 409B2 organoids at 58 days (region 3, 43 cells; region 4, 36 cells).
SummaryCerebral organoids – three-dimensional cultures of human cerebral tissue derived from pluripotent stem cells – have emerged as models of human cortical development. However, the extent to which in vitro organoid systems recapitulate neural progenitor cell proliferation and neuronal differentiation programs observed in vivo remains unclear. Here we use single-cell RNA sequencing (scRNA-seq) to dissect and compare cell composition and progenitor-to-neuron lineage relationships in human cerebral organoids and fetal neocortex. Covariation network analysis using the fetal neocortex data reveals known and novel interactions among genes central to neural progenitor proliferation and neuronal differentiation. In the organoid, we detect diverse progenitors and differentiated cell types of neuronal and mesenchymal lineages, and identify cells that derived from regions resembling the fetal neocortex. We find that these organoid cortical cells use gene expression programs remarkably similar to those of the fetal tissue in order to organize into cerebral cortex-like regions. Our comparison of in vivo and in vitro cortical single cell transcriptomes illuminates the genetic features underlying human cortical development that can be studied in organoid cultures.
Dataset viewGSE75140
PMID26644564

Samples in iPSC 409B2[35 days]

Displaying 31-40 of 68 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE75140GSM1957118Illumina HiSeq 2500Homo sapienswhOrg2_C6_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957119Illumina HiSeq 2500Homo sapienswhOrg2_C7_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957120Illumina HiSeq 2500Homo sapienswhOrg2_C8_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957121Illumina HiSeq 2500Homo sapienswhOrg2_C9_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957122Illumina HiSeq 2500Homo sapienswhOrg2_D11_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957123Illumina HiSeq 2500Homo sapienswhOrg2_D12_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957124Illumina HiSeq 2500Homo sapienswhOrg2_D1_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957125Illumina HiSeq 2500Homo sapienswhOrg2_D2_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957126Illumina HiSeq 2500Homo sapienswhOrg2_D3_hOrg35diPSC 409B2[35 days]
GSE75140GSM1957127Illumina HiSeq 2500Homo sapienswhOrg2_D4_hOrg35diPSC 409B2[35 days]

Gene rank in iPSC 409B2[35 days]

Displaying 21-30 of 16781 results.
Rank orderGene SymbolEnsembl ID
21DDX5ENSG00000108654
22ATP5BENSG00000110955
23TPI1ENSG00000111669
24TUBA1BENSG00000123416
25NAP1L1ENSG00000187109
26MARCKSENSG00000277443
27NPM1ENSG00000181163
28HIST1H4CENSG00000197061
29NGFRAP1
30TMSB4XENSG00000205542