scRNASeqDB - CellView Site

Overall Design	6 single human HepG2 cell line cells were sequenced using the newly developed scTrio-seq, other 2 HepG2 cells were sequenced using scRNA-seq and other 2 HepG2 cells were sequenced using scRRBS as technique control. 6 single mouse embryonic stem cells (mESCs) were sequenced using the newly developted scTrio-seq. Meanwhile, two scRNA-seq and two scRRBS were also completed using two mESCs separately. 26 single cells from hepatocellular carcinoma were sequenced using scTrio-seq to analyze the regulation relations between three omics of cancer cells.
Summary	Single cell genome, DNA methylome, and transcriptome sequencing has been achieved separately. However, to analyze the regulation of RNA expression by genetic and epigenetic factors within an individual cell, it is necessary to analyze these omics simultaneously from the same single cell. Here we developed a single cell triple omics sequencing technique- scTrio-seq, to analyze the genome, DNA methylome, and transcriptome concurrently of a mammalian cell.
Dataset view	GSE65364
PMID	26902283

Displaying 11-20 of 26 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE65364	GSM1593788	Illumina HiSeq 2000	Homo sapiens	Ca_11_RNA	liver cancer cell
GSE65364	GSM1593790	Illumina HiSeq 2000	Homo sapiens	Ca_12_RNA	liver cancer cell
GSE65364	GSM1593792	Illumina HiSeq 2000	Homo sapiens	Ca_13_RNA	liver cancer cell
GSE65364	GSM1593794	Illumina HiSeq 2000	Homo sapiens	Ca_14_RNA	liver cancer cell
GSE65364	GSM1593796	Illumina HiSeq 2000	Homo sapiens	Ca_15_RNA	liver cancer cell
GSE65364	GSM1593798	Illumina HiSeq 2000	Homo sapiens	Ca_16_RNA	liver cancer cell
GSE65364	GSM1593800	Illumina HiSeq 2000	Homo sapiens	Ca_17_RNA	liver cancer cell
GSE65364	GSM1593802	Illumina HiSeq 2000	Homo sapiens	Ca_18_RNA	liver cancer cell
GSE65364	GSM1593804	Illumina HiSeq 2000	Homo sapiens	Ca_19_RNA	liver cancer cell
GSE65364	GSM1593806	Illumina HiSeq 2000	Homo sapiens	Ca_20_RNA	liver cancer cell

Displaying 1-10 of 15622 results.