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  morula

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in morula

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160138Illumina HiSeq 2000Homo sapienshuman morula 1morula
GSE44183GSM1160139Illumina HiSeq 2000Homo sapienshuman morula 4morula
GSE44183GSM1160140Illumina HiSeq 2000Homo sapienshuman morula 5morula

Gene rank in morula

Displaying 14741-14750 of 14758 results.
Rank orderGene SymbolEnsembl ID
14741TMSB15BENSG00000158427
14742UPRTENSG00000094841
14743USP51ENSG00000247746
14744VCX2ENSG00000177504
14745XAGE3ENSG00000171402
14746XAGE5ENSG00000171405
14747XGENSG00000124343
14748XKENSG00000047597
14749ZC4H2ENSG00000126970
14750ZCCHC13ENSG00000187969