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  oocyte

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in oocyte

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160112Illumina HiSeq 2000Homo sapienshuman oocyte 1oocyte
GSE44183GSM1160113Illumina HiSeq 2000Homo sapienshuman oocyte 3oocyte
GSE44183GSM1160114Illumina HiSeq 2000Homo sapienshuman oocyte 4oocyte

Gene rank in oocyte

Displaying 41-50 of 14758 results.
Rank orderGene SymbolEnsembl ID
41RPL23ENSG00000125691
42FOXR1ENSG00000176302
43OAZ1ENSG00000104904
44C19orf53ENSG00000104979
45RPL35AENSG00000182899
46H1FOOENSG00000178804
47GDF9ENSG00000164404
48RPS25ENSG00000118181
49RPL9ENSG00000163682
50RPL12ENSG00000197958