scRNASeqDB - CellView Site

Overall Design	Total 213 H1 single cells and 247 H1-Fucci single cells were sequenced. The 213 H1 cells were used to evaluate Oscope in identifying oscillatory genes. The H1-Fucci cells were used to confirm the cell cycle gene cluster identified by Oscope in the H1 hESCs. Normalized expected counts are provided in GSE64016_H1andFUCCI_normalized_EC.csv.gz
Summary	Oscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets, include a single-cell RNA-seq data set of human embroyonic stem cells (hESCs), demonstrate advantages of the approach and also identify a potential artifact in the Fluidigm C1 platform.
Dataset view	GSE64016
PMID	26301841

Displaying 1-10 of 91 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE64016	GSM1562600	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.001	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562601	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.002	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562602	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.003	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562603	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.004	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562604	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.005	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562605	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.006	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562606	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.007	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562607	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.008	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562608	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.009	single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016	GSM1562609	Illumina HiSeq 2500	Homo sapiens	G1_Exp1.010	single H1-Fucci cell sorted from G1 phase of the cell cycle only

Displaying 11-20 of 17544 results.