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  single H1-Fucci cell sorted from G2-M phase of the cell cycle only

Overall DesignTotal 213 H1 single cells and 247 H1-Fucci single cells were sequenced. The 213 H1 cells were used to evaluate Oscope in identifying oscillatory genes. The H1-Fucci cells were used to confirm the cell cycle gene cluster identified by Oscope in the H1 hESCs. Normalized expected counts are provided in GSE64016_H1andFUCCI_normalized_EC.csv.gz
SummaryOscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets, include a single-cell RNA-seq data set of human embroyonic stem cells (hESCs), demonstrate advantages of the approach and also identify a potential artifact in the Fluidigm C1 platform.
Dataset viewGSE64016
PMID26301841

Samples in single H1-Fucci cell sorted from G2-M phase of the cell cycle only

Displaying 41-50 of 76 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE64016GSM1562820Illumina HiSeq 2500Homo sapiensG2_Exp1.042single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562821Illumina HiSeq 2500Homo sapiensG2_Exp1.043single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562822Illumina HiSeq 2500Homo sapiensG2_Exp1.044single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562823Illumina HiSeq 2500Homo sapiensG2_Exp1.045single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562824Illumina HiSeq 2500Homo sapiensG2_Exp1.046single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562825Illumina HiSeq 2500Homo sapiensG2_Exp1.047single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562826Illumina HiSeq 2500Homo sapiensG2_Exp1.048single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562827Illumina HiSeq 2500Homo sapiensG2_Exp1.049single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562828Illumina HiSeq 2500Homo sapiensG2_Exp1.050single H1-Fucci cell sorted from G2-M phase of the cell cycle only
GSE64016GSM1562829Illumina HiSeq 2500Homo sapiensG2_Exp1.051single H1-Fucci cell sorted from G2-M phase of the cell cycle only

Gene rank in single H1-Fucci cell sorted from G2-M phase of the cell cycle only