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  zygote

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in zygote

Displaying 1-2 of 2 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160118Illumina HiSeq 2000Homo sapienshuman zygote 1zygote
GSE44183GSM1160119Illumina HiSeq 2000Homo sapienshuman zygote 2zygote

Gene rank in zygote

Displaying 41-50 of 14758 results.
Rank orderGene SymbolEnsembl ID
41RPS23ENSG00000186468
42LDHBENSG00000111716
43RPL35AENSG00000182899
44RPL37ENSG00000145592
45RPL26ENSG00000161970
46RPS19ENSG00000105372
47RPL36ALENSG00000165502
48RPS14ENSG00000164587
49RANENSG00000132341
50UBA52ENSG00000221983