ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Pfn1

Full Name:

profilin 1

Alias:

Pfn

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

11 B4|11 42.0 cM

Physical Location:

70465350-70468136 on NC_000077.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 5216 Symbol (Name): PFN1 (profilin 1)

Ortholog Status:

The human GeneID 5216 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Dataset Information

Name:		16783646
Method:		Microarray and QTL
Tissue:		Whole brain
Phenotype:		Alcohol preference, acute functional tolerance
Publication:		Saba et al. Mamm Genome. (2006) Candidate genes and their regulatory elements: alcohol preference and tolerance. PubMed
Summary:		Identified of 8 candidate genes for the trait of alcohol preference and 22 candidate genes for acute functional tolerance

Gene Information

Original ID1:		Pfn1
Fold Change:		1.41
Note:		Significantly regulated by ethanol in cortical neurons

Gene Information

Original ID1:		Pfn1
Fold Change:		1.35
Note:		Significantly regulated in cortical neurons treated with both ethanol and nicotine

Dataset Information

Name:		17295719
Method:		Microarray
Tissue:		Cultured fetal mouse cortical neurons
Phenotype:		Ethanol response
Publication:		Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:		By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.

QTL Information

QTL	Phenotype	Chr	Peak (cM)	LOD	P Value	Range	Status	QTL Link
Lore4	Alcohol-induced Loss of Righting Reflex	11		36-42 (cM)	6.5		Significant	PARC

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_011072.3	NP_035202.1	NC_000077.5 range: 70465350..70468136, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0005634	nucleus	Cellular Component	IDA (15615774)
GO:0005737	cytoplasm	Cellular Component	IDA (11274401)
GO:0005856	cytoskeleton	Cellular Component	RCA (12466851)
GO:0015629	actin cytoskeleton	Cellular Component	RCA (12466851)
GO:0003779	actin binding	Molecular Function	RCA (12466851)
GO:0005515	protein binding	Molecular Function	IPI (11826110\|15615774)
GO:0001843	neural tube closure	Biological Process	IGI (10069337)
GO:0030036	actin cytoskeleton organization and biogenesis	Biological Process	IEA
GO:0008064	regulation of actin polymerization and/or depolymerization	Biological Process	TAS (11274401)
GO:0007010	cytoskeleton organization and biogenesis	Biological Process	RCA (12466851)
GO:0006357	regulation of transcription from RNA polymerase II promoter	Biological Process	IGI (15615774)
GO:0042989	sequestering of actin monomers	Biological Process	TAS (11274401)