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  colon carcinoma HCT116

Overall Design109 single-cell human transcriptomes were analyzed in total; 96 using nanoliter volume sample processing on a microfluidic platform, Nextera library prep (biological replicates); 3 using the SMARTer cDNA synthesis kit, Nextera library prep (biological replicates); 3 using the Transplex cDNA synthesis kit, Nextera library prep (biological replicates); 7 using the Ovation Nugen cDNA synthesis kit (biological replicates) where 3 used Nextera library prep and 4 used NEBNext library prep. In addition, 4 bulk RNA samples were sequenced: bulk RNA generated using ~1 million pooled cells was used to make bulk libraries, 2 of which were made using SMARTer cDNA synthesis kit (technical replicates) and 2 made using Superscript RT kit with no amplification (technical replicates). All 4 bulk samples were made into libraries using Nextera.
SummaryWe generated single-cell transcriptomes from a large number of single cells using several commercially available platforms, in both microliter and nanoliter volumes, and compared performance between them. We benchmarked each method to conventional RNA-seq of the same sample using bulk total RNA, as well as to multiplexed qPCR, which is the current gold standard for quantitative single-cell gene expression analysis. In doing so, we were able to systematically evaluate the sensitivity, precision, and accuracy of various approaches to single-cell RNA-seq. Our results show that it is possible to use single-cell RNA-seq to perform quantitative transcriptome measurements of individual cells, that it is possible to obtain quantitative and accurate gene expression measurements with a relatively small number of sequencing reads, and that when such measurements are performed on large numbers of cells, one can recapitulate the bulk transcriptome complexity, and the distributions of gene expression levels found by single-cell qPCR.
Dataset viewGSE51254
PMID24141493

Samples in colon carcinoma HCT116

Displaying 91-100 of 109 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE51254GSM1241231Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 78colon carcinoma HCT116
GSE51254GSM1241232Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 79colon carcinoma HCT116
GSE51254GSM1241233Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 80colon carcinoma HCT116
GSE51254GSM1241234Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 81colon carcinoma HCT116
GSE51254GSM1241235Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 82colon carcinoma HCT116
GSE51254GSM1241236Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 83colon carcinoma HCT116
GSE51254GSM1241237Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 84colon carcinoma HCT116
GSE51254GSM1241238Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 85colon carcinoma HCT116
GSE51254GSM1241239Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 86colon carcinoma HCT116
GSE51254GSM1241240Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 87colon carcinoma HCT116

Gene rank in colon carcinoma HCT116

Displaying 20311-20320 of 20378 results.
Rank orderGene SymbolEnsembl ID
20311C11orf88ENSG00000183644
20312CXorf36ENSG00000147113
20313LOC654433
20314A2ML1ENSG00000166535
20315LPAL2ENSG00000213071
20316MIR2110
20317SLC26A4ENSG00000091137
20318GLT25D2
20319CXorf21ENSG00000120280
20320BOLLENSG00000152430