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  hES hiPS-derived neuron

Overall DesignPatch-Seq of 20 hESC/hiPSC-derived neurons.
SummaryThe mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion are generated. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single-cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here, we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single-cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
Dataset viewGSE77564
PMID26883038

Samples in hES hiPS-derived neuron

Displaying 11-20 of 20 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE77564GSM2054562Illumina HiSeq 2500Homo sapiensSingle neuron 19hES hiPS-derived neuron
GSE77564GSM2054545Illumina HiSeq 2500Homo sapiensSingle neuron 2hES hiPS-derived neuron
GSE77564GSM2054563Illumina HiSeq 2500Homo sapiensSingle neuron 20hES hiPS-derived neuron
GSE77564GSM2054546Illumina HiSeq 2500Homo sapiensSingle neuron 3hES hiPS-derived neuron
GSE77564GSM2054547Illumina HiSeq 2500Homo sapiensSingle neuron 4hES hiPS-derived neuron
GSE77564GSM2054548Illumina HiSeq 2500Homo sapiensSingle neuron 5hES hiPS-derived neuron
GSE77564GSM2054549Illumina HiSeq 2500Homo sapiensSingle neuron 6hES hiPS-derived neuron
GSE77564GSM2054550Illumina HiSeq 2500Homo sapiensSingle neuron 7hES hiPS-derived neuron
GSE77564GSM2054551Illumina HiSeq 2500Homo sapiensSingle neuron 8hES hiPS-derived neuron
GSE77564GSM2054552Illumina HiSeq 2500Homo sapiensSingle neuron 9hES hiPS-derived neuron

Gene rank in hES hiPS-derived neuron

Displaying 19061-19070 of 19234 results.
Rank orderGene SymbolEnsembl ID
19061GGTA1PENSG00000204136
19062RNASELENSG00000135828
19063SLC25A51P1ENSG00000220483
19064OR9A2ENSG00000179468
19065LINC01029ENSG00000265843
19066MAGEA12ENSG00000213401
19067ANKRD34CENSG00000235711
19068KBTBD11-OT1ENSG00000253696
19069RAXENSG00000134438
19070ARHGEF38ENSG00000236699