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  iPSC-derived cortical neuron

Overall DesignSingle cell RNA-seq of 16 iPSC-derived cortical neurons.
SummaryInduced pluripotent stem cell (iPSC)-derived cortical neurons present a powerful new model of neurological disease. Previous work has established that differentiation protocols produce cortical neurons but little has been done to characterise these at cellular resolution. In particular, it is unclear to what extent in vitro two-dimensional, relatively disordered culture conditions recapitulate the development of in vivo cortical layer identity. Single cell multiplex RT-qPCR was used to interrogate the expression of genes previously implicated in cortical layer or phenotypic identity in individual cells. Unexpectedly, 22.7% of neurons analysed frequently co-expressed canonical fetal deep and upper cortical layer markers, and this co-expression was also present at the level of translated protein. By comparing our results to available single cell RNA-seq data from human fetal and adult brain, we observed that this co-expression of layer markers was also seen in primary tissue. These results suggest that establishing neuronal layer identity in iPSC-derived or primary cortical neurons using canonical marker genes transcripts is unlikely to be informative.
Dataset viewGSE69790
PMID26740550

Samples in iPSC-derived cortical neuron

Displaying 11-16 of 16 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE69790GSM1708825Illumina HiSeq 2000Homo sapiensC1_SM1_C52iPSC-derived cortical neuron
GSE69790GSM1708826Illumina HiSeq 2000Homo sapiensC1_SM1_C54iPSC-derived cortical neuron
GSE69790GSM1708827Illumina HiSeq 2000Homo sapiensC1_SM1_C64iPSC-derived cortical neuron
GSE69790GSM1708828Illumina HiSeq 2000Homo sapiensC1_SM1_C66iPSC-derived cortical neuron
GSE69790GSM1708829Illumina HiSeq 2000Homo sapiensC1_SM1_C79iPSC-derived cortical neuron
GSE69790GSM1708830Illumina HiSeq 2000Homo sapiensC1_SM1_C91iPSC-derived cortical neuron

Gene rank in iPSC-derived cortical neuron

Displaying 31-40 of 12561 results.
Rank orderGene SymbolEnsembl ID
31RPL15ENSG00000174748
32MLLT11ENSG00000213190
33CTTNENSG00000085733
34PTPRSENSG00000105426
35MIATENSG00000225783
36SETENSG00000119335
37FTLENSG00000087086
38SRSF6ENSG00000124193
39GPM6BENSG00000046653
40DYNC1H1ENSG00000197102