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  Primordial Germ Cells[male 19 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 19 week gestation]

Displaying 11-20 of 57 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677575Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc11Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677576Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc12Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677577Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc13Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677578Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc14Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677579Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc15Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677580Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc16Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677581Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc17Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677582Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc18Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677583Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc19Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677584Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc20Primordial Germ Cells[male 19 week gestation]

Gene rank in Primordial Germ Cells[male 19 week gestation]

Displaying 21301-21310 of 21483 results.
Rank orderGene SymbolEnsembl ID
21301TLR10ENSG00000174123
21302TLR8ENSG00000101916
21303TLX1ENSG00000107807
21304TLX1NBENSG00000236311
21305TMCO5BENSG00000215296
21306TMED11PENSG00000215367
21307TMED8ENSG00000100580
21308TMEM100ENSG00000166292
21309TMEM102ENSG00000181284
21310TMEM105ENSG00000185332