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  Primordial Germ Cells[female 17 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[female 17 week gestation]

Displaying 11-20 of 31 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677685Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc11Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677686Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc12Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677687Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc13Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677688Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc14Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677689Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc15Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677690Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc16Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677691Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc17Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677692Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc18Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677693Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc19Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677694Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc20Primordial Germ Cells[female 17 week gestation]

Gene rank in Primordial Germ Cells[female 17 week gestation]

Displaying 21411-21420 of 21483 results.
Rank orderGene SymbolEnsembl ID
21411VSX1ENSG00000100987
21412VTCN1ENSG00000134258
21413VTNENSG00000109072
21414VWA7ENSG00000204396
21415VWC2ENSG00000188730
21416WBP11P1ENSG00000260389
21417WDFY4ENSG00000128815
21418WDR49ENSG00000174776
21419WDR86ENSG00000187260
21420WFDC1ENSG00000103175