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  Primordial Germ Cells[male 10 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 10 week gestation]

Displaying 11-19 of 19 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677529Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc12Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677530Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc13Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677531Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc14Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677532Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc15Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677533Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc16Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677534Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc17Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677535Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc18Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677536Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc19Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677537Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc20Primordial Germ Cells[male 10 week gestation]

Gene rank in Primordial Germ Cells[male 10 week gestation]

Displaying 21271-21280 of 21483 results.
Rank orderGene SymbolEnsembl ID
21271VITENSG00000205221
21272VLDLR-AS1ENSG00000236404
21273VMACENSG00000187650
21274VMO1ENSG00000182853
21275VN1R10PENSG00000220758
21276VN1R2ENSG00000196131
21277VN1R4ENSG00000228567
21278VN1R5ENSG00000197617
21279VNN1ENSG00000112299
21280VNN2ENSG00000112303